Neuroscience 2003 Abstract
| Presentation Number: | 825.10 |
|---|---|
| Abstract Title: | Ethanol effects on synaptic transmission at crayfish neuromuscular junctions under different calcium concentrations. |
| Authors: |
Jensen, E. M.*1
; Velez, S. J.1
1Biological Sci., Dartmouth Col., Hanover, NH |
| Primary Theme and Topics |
Motor Systems - Invertebrate Motor Systems |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Synaptic Transmission<br />-- Presynaptic mechanisms |
| Session: |
825. Invertebrate Motor Systems Neuromodulation Poster |
| Presentation Time: | Wednesday, November 12, 2003 9:00 AM-10:00 AM |
| Location: | Morial Convention Center - Hall F-I, Board # H106 |
| Keywords: | muscle, endplate, crustacea, synaptic transmission |
Ethanol decreases synaptic transmission at the neuromuscular junction of the Superficial Flexor Muscle (SFM) system of the crayfish in a dosage-dependent manner (Jensen et.al., Program # 465.15, 2002 Abstract Viewer, SFN, 2002. CD-ROM). We continue studying the effects of ethanol on the SFM system of Procambarus clarkii by exposing the system to 2% ethanol under Ringers of varying calcium concentrations. Stable junction potentials (JPs) were recorded with microelectrodes while stimulating axon 6. The solution bathing the preparation was changed to one containing 2% ethanol in normal (100%), low (30%) or high (200%) calcium Ringers, and JP sizes were recorded every 3 min. for up to 18 min. before removing the drug. In experiments using normal Ringers before drug exposure, JPs decrease to 50% of their former size within 3 min. upon exposure to 2% ethanol in normal Ringers, and remain stable thereafter. They decline to less than 40% of their former value if they are exposed instead to 2% ethanol in either 30% or 200% calcium Ringers, achieving stable values by 9-18 min. In experiments using 30% or 200% calcium Ringers before drug exposure, JP sizes decreased to 50% of their former size within 3-9 min. of exposure to 2% ethanol in 30% or 200% calcium Ringers respectively. Thus it appears that as long as during solution changes calcium concentrations stayed constant, 2% ethanol had similar suppressive effects on JP sizes; if calcium concentrations are altered at the same time as ethanol is applied, 2% ethanol’s effects are modified. The data suggest that in this system ethanol is affecting transmitter release by acting at more than one place involved in regulating calcium homeostasis.
Supported by Dartmouth College Research Funds
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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