Neuroscience 2003 Abstract
| Presentation Number: | 37.9 |
|---|---|
| Abstract Title: | Type II PKA and its localization by AKAP150 is crucial for ocular dominance plasticity. |
| Authors: |
Fischer, Q. S.*1
; Yang, Y.1
; Jakobsdottir, K. B.1
; McKnight, G. S.2
; Daw, N. W.1
1Dept. Ophthalmology, Yale Univ. Sch. Med., New Haven, CT 2WA, 330 Cedar St., 06520-8061, |
| Primary Theme and Topics |
Development - Synaptogenesis and Activity-Dependent Development -- Activity-dependent development and plasticity |
| Session: |
37. Activity-dependent Development and Plasticity II Poster |
| Presentation Time: | Saturday, November 8, 2003 1:00 PM-2:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # C34 |
| Keywords: | CORTICAL PLASTICITY, MONOCULAR DEPRIVATION, PROTEIN KINASE A, VISUAL CORTEX |
PKA-RIIbeta-/- mice, despite compensatory upregulation of PKA-RI subunits, display little or no ocular dominance plasticity (ODP; Fischer et al., 2002, SFN abstr 820.9). We hypothesized that this occurs because RII subunits localize PKA to relevant substrates through binding with an A-kinase anchoring protein (AKAP). To test this hypothesis we examined ODP in: normal wild type, contralaterally deprived wild type, contralaterally deprived RIIbeta-/-, contralaterally deprived RIIalpha-/-, and contralaterally deprived AKAP150-/- mice. On postnatal day 24 (P24) deprived mice underwent monocular lid suture. Between P28-33 extracellular single-unit recordings were made in all mice to determine the weighted ocular dominance (WOD) scores of cells in several penetrations made across the medial-lateral extent of binocular V1. Genotypes were confirmed by PCR. WOD scores in contralaterally deprived RIIbeta-/- mice were significantly different from those in contralaterally deprived, but not normal wild type mice. In contrast, WOD scores in contralaterally deprived RIIalpha-/- and AKAP150-/- mice were significantly different from both normal and deprived wild type mice, indicating an incomplete blockade of ODP in these mutants. Finally, WOD scores of contralaterally deprived RIIalpha-/- and AKAP150-/- mice were not significantly different from each other or from contralaterally deprived RIIbeta-/- mice. Together these results show that RII-PKA and its localization, mediated at least in part by AKAP150, is crucial for ODP.
Supported by NEI R01 EY00053
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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