Neuroscience 2001 Abstract
| Presentation Number: | 881.16 |
|---|---|
| Abstract Title: | Gender Differences in Gene Expression in Human Brain Using Microarray and Model Based Analysis. |
| Authors: |
Vawter, M. P.*1
; Tomita, H.1
; Watson, S. J.2
; Akil, H.2
; Cox, D.4
; Jones, E. G.3
; Bunney, W. E.1
1Psychiatry & Human Behavior, University of California Irvine, Irvine, CA 2Mental Health Research Institute, University of Michigan, Ann Arbor, MI 3Center for Neuroscience, University of California, Davis, CA 4Genetics, Stanford University, Stanford, CA |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Genetic Models |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Genetic Models |
| Session: |
881. Genetic models I Poster |
| Presentation Time: | Wednesday, November 14, 2001 4:00 PM-5:00 PM |
| Location: | Exhibit Hall AAA-59 |
| Keywords: | PREFRONTAL CORTEX, CEREBELLUM, CINGULATE, DEPRESSION |
In our current dataset (12 samples) we compared human male and female gene expression levels on Affymetrix U95A chips using a model based expression analysis (Li and Wong, PNAS, 2001,98 (1),31-36). We replicated the findings of gender differences in gene expression (Evans, S. et al., SFN abstract this meeting). Our findings using a model based approach agreed with the Evans et al. findings on 4 of the 6 highest gene expression differences in which they used absolute Affymetrix calls as filtering criteria in pair wise comparisons between a larger set of male and female samples. We examined duplicate Affymetrix chips to establish reliability and false positive rates. The chip to chip variation suggested that two criteria were useful to minimize false positive gene expression differences. Thus, we find evidence of gene expression differences between gender that is specific to sex chromosome-linked genes. We have examined gene expression in three brain regions and find that two cortical regions, dorsolateral prefrontal cortex and anterior cingulate cortex, appear similar by clustering while cerebellum samples are separately clustered from cortical samples. We are determing whether differentially expressed genes in gender and brain regions are localized in neurons, glia, or all cells in the CNS. We will embark upon a microarray analysis of affective disorders using a case-control brain collection.
Supported by Conte Center Grant
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
Copyright © 2001-2026 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
