Neuroscience 2004 Abstract
| Presentation Number: | 31.12 |
|---|---|
| Abstract Title: | Subcutaneous administration of fibroblast growth factor-2 (FGF-2) enhances dentate neurogenesis in the injured aged hippocampus. |
| Authors: |
Hattiangady, B.*1,2
; Shetty, A. K.1,2
1Dept Surgery (Neurosurgery), Duke Univ Med Ctr, Durham, NC 2NC, Dept Surgery Div Neurosurg, 27710, |
| Primary Theme and Topics |
Development - Neurogenesis and Gliogenesis -- Neural stem cells |
| Secondary Theme and Topics | Development<br />- Neurogenesis and Gliogenesis<br />-- Neuronal differentiation |
| Session: |
31. Neurogenesis in the Dentate Gyrus I Poster |
| Presentation Time: | Saturday, October 23, 2004 4:00 PM-5:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # A1 |
| Keywords: | adult neurogenesis, aging, Alzheimer's disease, brain injury |
Hippocampal injury leads to increased addition of new neurons to the dentate granule cell layer (GCL) in the adult hippocampus but not in the middle-aged and the aged hippocampus (Rao et al., SFN Abstracts, 2003). We hypothesize that age-related malfunction in the response of dentate neurogenesis to hippocampal injury is owed to insufficient concentration of stem/progenitor cell proliferation factor FGF-2 in the injured aging hippocampus. To address this issue, we determined the number of new cells and neurons in the subgranular zone (SGZ) and GCL of the injured hippocampus of 24-months-old F344 rats following subcutaneous administration of fibroblast growth factor-2 (FGF-2). The hippocampal lesion was induced through an intracerebroventricular kainic acid administration, and FGF-2 was administered via daily subcutaneous injections between post-lesion days 4 and 15. The new cells and neurons that were born during FGF-2 administration were labeled using daily intraperitoneal injections of 5’-bromodeoxyuridine (BrdU; 100mg/Kg bw). Analyses at 10 days after the last BrdU injection using BrdU immunostaining, and BrdU/NeuN dual immunofluorescence revealed a clear dose-dependant increase in the addition of new neurons to the GCL of injured aged rats receiving FGF-2. In comparison to vehicle-treated rats, the increase was 135% in rats receiving low-doses of FGF-2 (40 ng/gm bw) and 262% in rats receiving higher doses of FGF-2 (60ng/gm bw). Thus, stem/progenitor cells in the SGZ of the injured aged hippocampus can be stimulated to produce more new neurons by simple subcutaneous injections of FGF-2. These results have implications toward development of therapeutic strategies for neurodegenerative diseases afflicting the aging hippocampus, particularly the Alzheimer's disease.
Supported by NIA Grant RO1 AG20924 to A.K.S.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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