Neuroscience 2005 Abstract
| Presentation Number: | 783.15 |
|---|---|
| Abstract Title: | Quantitative analysis of spinal cord contusion injury and locomotor recovery in rats with diffusion-weighted MRI. |
| Authors: |
Ameenuddin, S.*1
; Dinca, E.1
; Endo, T.1
; Mishra, P. K.1
; Macura, S. I.1
; Hanson, D. P.1
; Gross, L.1
; Koch, A.1
; Spinner, R. J.1
; Marsh, W. R.1
; Yaszemski, M. I.1
; Windebank, A. J.1
1Molecular Neuroscience Program, Mayo Clinic Col. of Medicine, Rochester, MN |
| Primary Theme and Topics |
Disorders of the Nervous System - Trauma -- Spinal cord: Animal models and human studies |
| Session: |
783. Spinal Cord Injury: Models Poster |
| Presentation Time: | Tuesday, November 15, 2005 3:00 PM-4:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # SS50 |
| Keywords: | spinal cord injury, MRI, difffusion -weighted MRI, functional recovery |
To determine the extent of spinal cord injury over time in rats, we are using noninvasive techniques, including conventional MRI and diffusion-weighted MRI, to evaluate the integrity of the spinal cord, the volume of injury and inflammatory changes. Adult female Sprague-Dawley rats were injured at the T9 level using the NYU impactor (10 g from 50mm on to the intact spinal cord). Locomotor behavior was recorded weekly using BBB score. At the end of 2, 7, 14 and 28 days non-fixed spinal columns were placed into a 10mm bore glass tube for MRI analysis using a 7 Tesla vertical bore magnet with the Bruker Biospin®10mm MICRO imaging probe and ParaVision 3.0.2® image acquisition software. Seven 1mm thick coronal and sagittal slices and twenty-three 1mm thick axial slice images were captured to show the entire region of interest in the spinal cord. The in-plane image resolution of the coronal and sagittal slices was 39µm/pixel and in the axial slices was 43µm/pixel. Multi-slice spin echo images at TR = 2100ms, TE = 21ms complement the diffusion weighted images using a diffusion gradient strength of 160 mT/m perpendicular to the spinal cord axis with TR = 2500ms, TE = 52ms. The extent of the lesion was measured using ANALYZE 6.1® image analysis software. Tissue was post-fixed in 4% paraformaldehyde, re-imaged and then processed for histological analysis to confirm the lesion size, and the morphological changes in gray and white matter. The extent of injury after contusion was significantly reduced over time (9.4, 8.0, 7.6 and 5.8 mm3 ) and correlated well with improved behavioral recovery (BBB score: 3.9, 8.4,11.7 and 13.3) for 2, 7, 14 and 28 days respectively. These techniques provide accurate non-invasive measurements for evaluating the underlying structural damage to the spinal cord. In these studies, diffusion-weighted MRI did not provide additional information.
Supported by Mayo Foundation & NIH (EB2390)
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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