Neuroscience 2000 Abstract
| Presentation Number: | 797.1 |
|---|---|
| Abstract Title: | Motoneuron regeneration into nerve grafts with competing axons from motor and sensory nerves. |
| Authors: |
Midha, R.*1
; Sulaiman, O. A. R.2,3
; Munro, C. A.1
; Gordon, T.2,3
1Div of Neurosurg, Univ of Toronto, Toronto, Canada 2Div of Neurosci, Univ of Alberta, Edmonton, Canada 3Dept of Pharmacol, Univ of Alberta, Edmonton, Canada |
| Primary Theme and Topics |
A. Development and Regeneration - 22. Regeneration |
| Session: |
797. Regeneration XI Poster |
| Presentation Time: | Thursday, November 9, 2000 8:00 AM-9:00 AM |
| Location: | Hall G-J |
| Keywords: | peripheral nerve, nerve autograft, femoral nerve, peripheral nerve regeneration |
Femoral motoneuronal(M) regeneration into saphenous nerve grafts(G) that are reinnervated simultaneously at the distal end with sensory axons(S) (MGS group), is 50% reduced compared to motoneuronal regeneration in grafts that are ligated distally (MGL group, Midha et al, SFN 1999). This maybe due to competition between sensory and motor axons for endoneurial pathways or an inhibitory influence of sensory axons on motoneuronal regeneration in sensory pathways. To test this, we evaluated the ability of motoneurons to regenerate into sensory nerve grafts at the same time as motoneurons regenerated into the graft from the distal end. In a rat model, a 4 cm saphenous autograft was interposed and sutured to the proximal stumps of the cut motor branches of both the left and right femoral nerves(MGM, n=4). 3 months later, the graft was sectioned in the centre, each end of the graft was exposed to 1 of 2 retrograde tracers (fluorogold or fluororuby). Rats were perfused 5 day later, the T11-L2 spinal segment removed, 50 um longitudinal sections cut and femoral motoneurons counted using fluorescent microscopy. Motoneuronal regeneration from the left (135±17.6, mean ±SD) into the graft, was similar to that from the right side (134±26.5). The MGM counts were significantly greater (t=4.02, p<.001) than the MGS (98.9±5.9). MGM counts did not differ from the MGL (150.6±12.2). These results indicate that competition by axons alone does not inhibit motoneuron regeneration. The presence of sensory axons exerts an inhibitory influence on motoneuronal regeneration, which maybe from axonal or Schwann cell induced factors.
Supported by MRC and PSI
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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