Neuroscience 2003 Abstract
| Presentation Number: | 673.8 |
|---|---|
| Abstract Title: | Expression and functional analysis of a novel protein kinase, Ebr kinase, in developing mouse brain. |
| Authors: |
Matsuki, T.*1
; Furuichi, T.1
1Lab. Molec Neurogenesis, RIKEN, Wako, Japan |
| Primary Theme and Topics |
Development - Neurogenesis and Gliogenesis -- Neuronal differentiation |
| Session: |
673. Gene Expression in Development Poster |
| Presentation Time: | Tuesday, November 11, 2003 4:00 PM-5:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # A50 |
| Keywords: | DEVELOPMENT, MICROARRAY, MIDBRAIN |
We previously reported the results of our gene expression profiling analysis of embryonic mouse brains utilizing the Affymetrix GeneChip (SFN Annual Meeting, 2001). We recently identified a novel protein kinase gene by in silico and experimental cDNA cloning of differentially expressed gene candidates obtained by the GeneChip analysis, and designated as Ebr kinase (embryonic brain kinase). The Ebr kinase gene encodes 435 amino acids and contains Ser/Thr protein kinase domain. To confirm the temporal developmental expression profiles of Ebr kinase gene, we subjected it to a more reliable, real time quantitative RT-PCR analysis using the TaqMan system. The data obtained by both GeneChip and TaqMan PCR methods showed almost parallel down-regulation profile. We investigated the activity of Ebr kinase was investigated by in vitro kinase assay using GST fusion proteins of wild type and kinase domain mutants, and indicated that the wild type had kinase activity whereas the mutants did not it. Next, we examined the spatial cellular expression patterns by whole mount and section in situ hybridization and immunohistochemistry. At E12, the Ebr kinase gene was almost exclusively expressed in the brain and predominantly in the telencephalon, mesencephalon, and metencephalon. Interestingly, the strongest immunoreactivity was observed in the marginal zone of the mesencephalon at E12. In E15 mouse brains, expression of the Ebr kinase gene occurred predominantly in the olfactory bulb and telencephalon. In the telencephalon, the Ebr kinase gene was more highly expressed in the cortical plate than in the ventricular zone. In the cerebellum, Ebr kinase gene showed low level expression in the external germinal layer and rhombic lip that corresponded to the postmitotic migrating zone and mitotic zone of cerebellar granule cell precursors, respectively. These results suggest that Ebr kinase may play differential roles in signal transduction during neuronal differentiation and/or proliferation in embryonic brains.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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