Neuroscience 2004 Abstract
| Presentation Number: | 654.6 |
|---|---|
| Abstract Title: | Analysis of lesion volume by MRI and histological analysis in C57Bl/6 mice with various levels of T9 spinal cord contusion injuries. |
| Authors: |
Nishi, R. A.*1
; Liu, H.1
; Cadena, G.1
; Thamkruphat, T.1
; Chu, Y.4
; Hamamura, M. J.4
; Su, M.4
; Nalcioglu, O.4
; Anderson, A. J.1,2,3
1CRPF SCI Core, UC Irvine, Irvine, CA 2Physical Med & Rehab., UC Irvine, Irvine, CA 3Anat. & Neurobiology, UC Irvine, Irvine, CA 4Radiological Sci., UC Irvine, Irvine, CA |
| Primary Theme and Topics |
Motor Systems - Spinal Cord -- Damage and recovery |
| Secondary Theme and Topics | Motor Systems<br />- Spinal Cord<br />-- Imaging |
| Session: |
654. Spinal Cord Injury: Models Poster |
| Presentation Time: | Tuesday, October 26, 2004 9:00 AM-10:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # EE13 |
| Keywords: | STEREOLOGY, FIBRONECTIN, POLYESTER WAX, SPINAL CORD INJURY |
We are investigating the feasibility and reliability of using MRI as a method to accurately measure lesion volume in the spinal cord of contusion-injured mice. We compared the results of lesion volumes determined using T-1 weighted images in a 3-D gradient echo sequence on a 7T MRI with lesion volumes determined by stereological sampling of fibronectin immunoreactivity in sections processed from the same spinal cords. Histology was performed on 10 µm polyester wax-embedded sections, using the Cavalieri method. Eight week old female C57Bl/6 mice received a mild (30 kDyne), moderate (50 kDyne), or severe (70 kDyne) contusion injury at the T9 vertebral level using the IH Impactor (Infinite Horizons). Control mice received a T9 laminectomy only. Mice were tested at 3 days, and weekly after the injury for functional recovery with the BBB (Basso, Beattie, Bresnahan) and BMS (Basso Mouse Scale) locomotor rating scales. Eight weeks after injury, mice were perfused with 4% paraformaldehyde, the spinal cord collected between the T6 and T12 spinal roots, and stored in PBS at 4°C until MRI analysis. Preliminary analysis shows that lesion volume can be detected using this MRI method. We will show the comparison of lesion volumes analyzed by MRI and stereology. Future studies are planned to further refine the speed and resolution of the MRI scanning, in order to develop optimal cost vs. time and resolution parameters to allow accurate determination of lesion volumes and tissue sparing. These results will give researchers more options in how to analyze spinal cord injures in animal models.
Supported by Christopher Reeve Paralysis Foundation
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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