Neuroscience 2004 Abstract
| Presentation Number: | 619.7 |
|---|---|
| Abstract Title: | Combination therapy of neuronal and glial restricted precursors (NRP/GRP) transplants with tamsulosin, an α1a-adrenergic antagonist, improves bladder function in spinal cord injury rats. |
| Authors: |
Mitsui, T.*1
; Shumsky, J. S.1
; Amato, N. M.1
; Hanford, K. C.1
; Murray, M.1
; Fischer, I.1
1Neurobiol. and Anat., Drexel Univ. Col. of Med., Philadelphia, PA |
| Primary Theme and Topics |
Development - Transplantation and Regeneration -- Regeneration: CNS |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Trauma<br />-- Spinal cord |
| Session: |
619. Regeneration CNS: Spinal Cord II Poster |
| Presentation Time: | Tuesday, October 26, 2004 10:00 AM-11:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # G30 |
| Keywords: | SPINAL CORD INJURY, BLADDER, NEURAL STEM CELLS, SPROUTING |
Bladder dysfunction is a consequence of spinal cord injury that can be ameliorated by cellular transplantation (Mitsui et al. 2003-SfN). Here we examined effects of a different cellular transplant (NRP/GRP) alone and in combination with pharmacotherapy (Tamsulosin) on recovery of bladder function. Female rats received a modified moderate contusion injury at T8/9, and mixed NRP/GRP or medium alone (OP-controls) were delivered into the injury as well as caudal and rostral at 9 days post-injury. NRP/GRP transplants promoted a significant increase in voided volume/micturition at 2 wk while OP-controls reached the same level at 4 wk, indicating an accelerated recovery. Cystometry studies at 8 wk revealed a decreased micturition pressure and fewer episodes of detrusor hyperreflexia in NRP/GRP, but no differences in post-void residual urine or bladder capacity. We also found that NRP/GRP transplants partially prevented bladder hypertrophy. Thus, NRP/GRP transplants promote functional recovery similar to that shown by transplants of fibroblasts genetically modified to secrete BDNF/NT-3. When Tamsulosin (10μg/animal) was administered via intrathecal catheter at L6-S1 following baseline cystometric measures, we observed improvement in all cystometric parameters. Immunocytochemical analysis of several axonal markers at L6-S1 showed increased density of 5-HT, CRF and DBH-positive fibers in the NRP/GRP group, suggesting that the graft induced sprouting of several descending pathways. Density of CGRP, VR1 and GAP43 staining was less in the NRP/GRP group, suggesting that the graft inhibited sprouting of dorsal root axons. Thus, NRP/GRP transplants improve recovery of bladder function following spinal cord injury and the combination with Tamsulosin further improves urodynamic parameters.
Supported by NS24707, SCRF, Uehara Memorial Foundation
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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