Neuroscience 2004 Abstract
| Presentation Number: | 581.6 |
|---|---|
| Abstract Title: | Presence of endogenous agmatine in opioid tolerance and addiction-related CNS regions in rodents. |
| Authors: |
Goracke-Postle, C. J.*1
; Nguyen, H. X.2,3
; Overland, A. C.1,2
; Roberts, J. C.1,2
; Morgan, A. D.1,2,4
; Kitto, K. F.1,2,3
; Grocholski, B. M.2,3
; Fairbanks, C. A.1,2,3
1Dept. and Grad. Program in Neurosci., Univ. of Minnesota, Minneapolis, MN 2Pharmaceutics, Univ. of Minnesota, Minneapolis, MN 3Pharmacol., Univ. of Minnesota, Minneapolis, MN 4Psychiatry, Univ. of Minnesota, Minneapolis, MN |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Addiction and Drugs of Abuse -- Neuroplasticity and addiction |
| Session: |
581. Neuroplasticity of Addiction II Poster |
| Presentation Time: | Monday, October 25, 2004 2:00 PM-3:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # FFF27 |
| Keywords: | self-administration, hyperalgesia, arginine, immunoneutralization |
Agmatine reduces opioid-induced tolerance and self-administration. Conversely, anti-agmatine IgG sensitizes rodents to these phenomena (Morgan-AD, SfN 2004). We evaluated the presence of endogenous agmatine in regions that drive these events. HPLC analysis was conducted on mouse CNS tissue and serum extracted following intrathecal and intracerebroventricular administration of agmatine. The concentration-time profile and the duration of agmatine pharmacodynamics were determined. Additionally, the localization of agmatine in spinal cord and in nucleus accumbens was conducted using selective anti-agmatine IgG. Effects of agmatine immunoneutralization were also evaluated. After intrathecal delivery peak agmatine concentrations were detected in spinal cord within 6 hours. An apparent elimination phase was evident up to 3 days. Intrathecal agmatine inhibition of NMDA-evoked pharmacodynamic measures was maximal at co-injection and ineffective at five minutes. Following ICV administration peak agmatine concentration was detected in nucleus accumbens within 15 minutes; the elimination phase was apparent by 60 minutes. Using laser confocal microscopy, we detected agmatine-like immunoreactivity in spinal cord and in the nucleus accumbens that was concentration dependently pre-absorbed by agmatine. Anti-agmatine IgG concentration dependently prevented agmatine-mediated inhibition of NMDA-evoked responses. The evidence presented here suggests that endogenous agmatine is located where it could contribute to modulation of plasticity-mediated events.
Supported by NIDA T32 DA07097 (CJG-P), K01 DA-00509(CAF) and R21 DA15387 (CAF).
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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