Neuroscience 2005 Abstract
| Presentation Number: | 587.7 |
|---|---|
| Abstract Title: | <i>In vivo</i> MRI detection of amyloid plaques in AD transgenic using gadolinium labeled non-toxic Aâ homologous ligands followed by voxel-based analysis. |
| Authors: |
Wisniewski, T.*1,2,3
; Mosconi, L.3
; Wadghiri, Y.5
; Blind, J. A.5
; Tsui, W.3
; Knudsen, E.3
; Asuni, A.3
; Sadowski, M.1
; Turnbull, D. H.2,4,5
; de Leon, M.3
; Sigurdsson, E.3
1Neurology, New York Univ. School of Medicine, New York, NY 2Pathology, New York Univ. School of Medicine, New York, NY 3Psychiatry, New York Univ. School of Medicine, New York, NY 4Radiology, New York Univ. School of Medicine, New York, NY 5NY, Millhauser Lab, HN 419, 10016, |
| Primary Theme and Topics |
Disorders of the Nervous System - Neurodegenerative and Movement Disorders -- Alzheimer's disease: APP and presenilin -- abeta |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, Tracing, and Imaging Techniques |
| Session: |
587. Abeta IV Slide |
| Presentation Time: | Tuesday, November 15, 2005 9:30 AM-9:45 AM |
| Location: | Washington Convention Center - Room 151B |
| Keywords: | Alzheimer's Disease, Imaging |
We have previously reported in vivo µMRI detection of plaques following intra-carotid injection of Gd-DTPA-Aß1-40 in AD model mice. However, Aß1-40 is toxic and can potentially seed amyloid plaque growth. In the present study we developed non-toxic, Aß homologous probes, which can detect early amyloid plaques in vivo. Furthermore, we examined the MRI scans using voxel-based analysis, to allow for un-biased qualitation. µMRI was performed in AD Tg mice and wild-type controls prior to injection and following intra-carotid and intravenous injection with gadolinium-DTPA-K6Aß1-30. Imaging scans were realigned, spatially normalized to match the shape of a standard template MRI image in stereotactic space, smoothed and examined for statistical differences using statistical parametric mapping (SPM, version 2002) . Large plaques were detected in vivo prior to injection of contrast agent. Using Perl's staining these large plaques contained extensive iron deposits. Following injection, the contrast of these plaques was enhanced and additional smaller plaques were observed. Statistical parametric mapping comparing pre-ligand and post-ligand injection images showed significant increases in amyloid detection in cortical and hippocampal regions (p<0.05, corrected for multiple comparisons). Our studies show that using our imaging parameters: 1) Large plaques can be detected without the use of ligand and this detection correlates with the presence of iron deposition. 2)Smaller, earlier plaques require contrast ligand for detection. 3) Gadolinium-DTPA-K6Aß1-30 is an effective non-toxic, ligand for plaque detection. 4) Voxel-based analysis using SPM is an effective way to compare pre and post ligand injection images.
Supported by NIH AG20245
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
Copyright © 2005-2026 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
