Neuroscience 2005 Abstract
| Presentation Number: | 569.1 |
|---|---|
| Abstract Title: | Development of EPR/MRI dual imaging system as a tool to detect bioradicals. |
| Authors: |
Fujii, H.*1
; Sakata, M.2
; Itoh, k.3
1School of Health Sciences, Sapporo Med. Univ., Sapporo, Japan 2School of Medicine, Sapporo Med. Univ., Sapporo, Japan 3Japan, South-1, West-17, Chuo-ku, 060-8556, |
| Primary Theme and Topics |
Techniques in Neuroscience - Physiological Methods |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Epilepsy<br />-- Basic mechanisms |
| Session: |
569. Physiological Techniques Poster |
| Presentation Time: | Monday, November 14, 2005 1:00 PM-2:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # WW3 |
| Keywords: | Free radical, EPR, MRI, Imaging |
Speculations about the role of free radicals generated in biological systems (so called bioradicals) are commonplace in many recent publications dealing with oxidative stress, inflammation and hypoxia reperfusion. Electron Paramagnetic Resonance Imaging (EPRI) technique has been developed to obtain the spatial distribution of the paramagnetic materials, particularly on a microscopic scale. A large number of biological studies were successfully carried out, especially for the visualization of bioradicals generated in vivo system. Typical example is the detection and visualization of nitric oxide (NO) in septic-shocked animals. The strength of EPRI is that only free radicals are visible in its image. On the contrary, weakness of EPRI is that nothing can be visible except paramagnetic materials on its image. Therefore, it seems imposible to predict in which organs the bioradicals were generated most from the results with EPRI. In order to overcome this problem, in this study, we have started developing EPR/MRI dual imaging system to visualize free radicals and paramagnetic materials on the anatomical map of the object. EPR/MRI dual imaging system was constructed by using the permanent magnet (Nb-Fe-B material) rather than electromagnets or superconductive magnets because it is easier to assemble and combine EPR spectrometer and MRI components. EPR spectrometer in this dual imaging system is working at 1.2 GHz at 0.04T, and MRI instrument in this dual system is working at 22 MHz at 0.5T. In the dual imaging system, 0.5 T magnet for MRI was installed with 0.04 T magnet for EPR, in which each center was only 20 cm apart. Each spectrometer was working well without any magnetic or electric interference. MR images of mice doped with paramagnetic drugs were successfully obtained, and soon after that EPRI and spectra of the paramagnetic drugs doped into mice can be obtained.
Supported by Grant-in-Aid for Scientific Research from JSPS, Japan
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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