Neuroscience 2005 Abstract
| Presentation Number: | 568.6 |
|---|---|
| Abstract Title: | <sup>1</sup>H magnetic resonance spectroscopy of the mouse hippocampus. |
| Authors: |
Boska, M. D.*1,3
; Nelson, J. A.1,3
; Yamamoto, M.2,3
; Mosley, R. L.2,3
; Gendelman, H. E.2,3
; Ikezu, T.2,3
1Radiology, Univ. of Nebraska Med., Omaha, NE 2Pharmacology and Experimental Neuroscience, Univ. of Nebraska Med., Omaha, NE 3Center for Neurovirology and Neurodegenerative Disorders, Univ. of Nebraska Med., Omaha, NE |
| Primary Theme and Topics |
Techniques in Neuroscience - Mass Spec and Other Biochemical and Analytical Methods |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Neurodegenerative and Movement Disorders<br />-- Other |
| Session: |
568. Mass Spectometry and Other Biochemical and Analytical Methods Poster |
| Presentation Time: | Monday, November 14, 2005 2:00 PM-3:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # VV72 |
| Keywords: | IMAGING, METABOLISM, HIPPOCAMPUS |
1H magnetic resonance spectroscopic imaging (1H MRSI) is a sensitive and early indicator of metabolic nervous system dysfunction. Accounting for spatial dependency of spectroscopic signals will improve 1H MRSI diagnostic precision. Thus, hippocampal and striatal spectra were compared. Methods: Mouse brain 1H MRSI was acquired with a Bruker 7T/21 MRI/MRS system. Spectroscopic images were recorded using 20 X 20 phase encoding over a 20 mm field of view using 1 mm slice thickness, 50 ms TE and 4 s TR. We assessed (Figure L) MRI images corresponding to the center of the selected slice and spectra from single voxels corresponding to the compiled mouse brain regions. Analyses (Figure-R) of the ratios of N-acetyl aspartate (NAA) choline (Cho), myoinostitol (mI), glutamate (Glu) and glutamine (Gln) normalized to the creatine (Cre) peak from within each spectrum were performed.
Conclusions: NAA/Cre, Cho/Cre, and mI/Cre are spatially dependent, with reductions from the CA1 to CA3 hippocampal regions. Glu/Cre increases over the same areas, whereas Gln/Cre remains consistent within measurement error. Similar spatial variations of NAA/Cre were previously reported for humans (1). These approaches are now being applied to studies of trymethyltin-induced hippocampal neurodegeneration in mouse models of beta-amyloid deposition.
References:1: Hetherington, HP, et al, Epilepsia, 45(Suppl. 4):1–7, 2004.
Conclusions: NAA/Cre, Cho/Cre, and mI/Cre are spatially dependent, with reductions from the CA1 to CA3 hippocampal regions. Glu/Cre increases over the same areas, whereas Gln/Cre remains consistent within measurement error. Similar spatial variations of NAA/Cre were previously reported for humans (1). These approaches are now being applied to studies of trymethyltin-induced hippocampal neurodegeneration in mouse models of beta-amyloid deposition.
References:1: Hetherington, HP, et al, Epilepsia, 45(Suppl. 4):1–7, 2004.
Supported by NIH grants 2R37NS36136, P20RR15635, P01 NS31492, P01 MH050244, and the Vada Oldfield Award.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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