Neuroscience 2005 Abstract
| Presentation Number: | 563.11 |
|---|---|
| Abstract Title: | Discovery of the presence and functional expression of CB<sub>2</sub> cannabinoid receptors in brain that is involved in depression and substance abuse. |
| Authors: |
Onaivi, E. S.*1,3
; Hiroki, I.2
; Gong, J.3
; Patel, S.1
; Meozzi, P.1
; Myers, L.1
; Mora, Z.1
; Perchuk, A.1
; Tagliaferro, P.
; Leonard, C.1
; Gardner, E.1
; Brusco, A.
; Akinshola, B. E.
; Liu, Q.3
; Hope, B.
; Uhl, G. R.3
1William Paterson Univ., Wayne, NJ 2Japan, 300 Pompton Rd, 07470, 3Basic Medical Sciences, 300 Pompton Rd, 07470, |
| Primary Theme and Topics |
Disorders of the Nervous System - Addiction and Drugs of Abuse -- Cannabinoids |
| Secondary Theme and Topics | Neural Excitability, Synapses, and Glia: Cellular Mechanisms<br />- G-Protein Linked Receptors<br />-- Other |
| Session: |
563. Cannabinoids Poster |
| Presentation Time: | Monday, November 14, 2005 3:00 PM-4:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # UU80 |
| Keywords: | Reward, Stress, RT-PCR, Neuroinflammation |
Two well-characterized cannabinoid receptors (CBrs), CB1 and CB2 mediate the effects of cannabinoids and marijuana use. CB1 receptors are expressed primarily in the brain and in peripheral tissues. Several laboratories have not been able to detect CB2 in brain that are intensely expressed in peripheral and immune tissues and has traditionally been referred to as peripheral CB2 CBrs. In mice the effects of direct CB2 antisense olignucleotide injection into the brain and i.p treatment with JWH015 in motor function and plus-maze tests were evaluated. We also used RT-PCR, immunoblotting, immunohistochemistry, and hippocampal cultures to determine the expression of CB2 CBrs in rat brain and in mice brain exposed to chronic mild stress (CMS) or those treated with cocaine or heroin. JWH015 a CB2 receptor agonist reduced mouse locomotor activities while direct CB2 antisense oligonucleotide microinjection induced anxiolysis. In the CMS animals the expression of CB2 CBrs was enhanced and was modified in the brains of cocaine and heroin treated rats. Abundant iCB2 in neuronal and glial processes were detected in brain and CB2 expression was detected in NSE positive hippocampal cell cultures. Contrary to the prevailing view that CB2 CBrs is restricted to peripheral tissues and predominantly in immune cells, we demonstrate that CB2 CBrs and their gene transcripts are widely distributed in the brain. The presence and functional expression of CB2 CBrs in the brain supports broader CNS roles for this receptor and may be exploited as new target in the treatment of depression and substance abuse.
Supported by NIDA and WPUNJ CFR
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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