Neuroscience 2001 Abstract
| Presentation Number: | 591.7 |
|---|---|
| Abstract Title: | EFFECT OF MATERNAL LIPOPOLYSACCHARIDE EXPOSURE ON GENE EXPRESSION IN THE FETAL MOUSE BRAIN. |
| Authors: |
Kaftan, H. A.*1
; Cui, L.2
; Choudhuri, R.2
; Klein, R. M.2
; Berman, N. E. J.2
1Pediatrics, Children's Mercy Hospital, Kansas City, MO 2Anatomy & Cell Biology, University of Kansas Medical Center, Kansas City, KS |
| Primary Theme and Topics |
Development - Axonal and Dendritic Development |
| Session: |
591. Axonal and dendritic development: axon growth and guidance--semaphorins, netrins, ephrins, CAMs Poster |
| Presentation Time: | Tuesday, November 13, 2001 3:00 PM-4:00 PM |
| Location: | Exhibit Hall B-55 |
| Keywords: | necdin, semaphorin g, groucho |
There is growing evidence that maternal infection and the subsequent induction of proinflammatory cytokines play a role in fetal brain injury. It has been noted that children with cerebral palsy have higher serum concentration of proinflammatory cytokines at birth than those of controls. Animal models of maternal infection have shown increased expression of TNF-α, IL-6, and IL-1 β in the fetal brain. We have reported increased expression of MCP-1 mRNA in the fetal brain of mice after maternal exposure to LPS(SFN Abstr 26:1619, 2000). While it is becoming clear that inflammation plays a role in periventricular white matter injury, the final pathway and the primary cells injured remains unclear. To further study this question, we gave an intraperitoneal injection of 50 µg of E.coli LPS to C57X129 pregnant mice at day 18 of gestation. Fetal brains were isolated and frozen in liquid nitrogen for RNA extraction. The differential gene expression of a variety of proteins was analyzed by using the Atlas mouse 1.2 DNA array from CLONTECH and RT-PCR. When compared to control mice, there was increased TNF-α and decreased expression of semaphorin g, groucho, and necdin genes in the brain of mice exposed to LPS in utero. Semaphorin g plays a role in axon generation and migration. Groucho is involved in the patterning of neurogenesis. Necdin is a cell cycle regulator expressed exclusively in postmitotic cells and therefore, is a marker of differentiated neurons. Disruption of these genes may play a role in the development of periventricular white matter injury.
Supported by CMH and NS38202
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
Copyright © 2001-2026 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
