Neuroscience 2002 Abstract
| Presentation Number: | 591.18 |
|---|---|
| Abstract Title: | Demonstration of distinct complements of three or four repeat domain isoforms of tau in tauopathy inclusions using novel specific monoclonal antibodies. |
| Authors: |
de Silva, R.*1,2
; Lashley, T.2
; Strand, C.2
; Gibb, G.3
; Hanger, D.3
; Hope, A.1
; Reid, A.1
; Bandopadhyay, R.1
; Utton, M.3
; Anderton, B.3
; Wood, N.2
; Holton, J.2
; Revesz, T.2
; Lees, A.1,2
1Reta Lila Weston Inst of Neurol Studies, Univ College Med. School, LONDON, United Kingdom 2Dept of Molecular Pathogenesis, Institute of Neurology, LONDON, United Kingdom 3Dept of Neurosciences, Institute of Psychiatry, LONDON, United Kingdom |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Neurodegenerative Disorders -- Alzheimers Disease: Tau |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, tracing and imaging techniques |
| Session: |
591. Neurodegenerative disorders: Alzheimer's disease--tau II Poster |
| Presentation Time: | Tuesday, November 5, 2002 2:00 PM-3:00 PM |
| Location: | Hall A2-B3 V-27 |
| Keywords: | TAU, PARKINSON, ANTIBODY |
Tau exon 10 splice site mutations in FTDP-17 result in the relative increase of tau isoforms containing four microtubule binding repeats (4R-tau) over three repeat isoforms (3R-tau). This is considered a fundamental process in disease pathogenesis. Although other tauopathies including PSP, CBD, PiD and FTDP-17 with tau missense mutations are not primarily caused by the same mechanism, insoluble, fibrillar tau in the pathological inclusions in these disorders are classified by differences in their tau isoform profiles. In order to study the relevance of these isoform differences in selective neuonal death, we have developed specific monoclonal antibodies, RD3 and RD4 that recognise the 3R- and 4R-tau, respectively. Using these, we have identified changes in the ratio and cellular distribution of these isoforms, and compare these changes in the different tauopathies. These findings could give us insight into the possible basis of the phenotypic range of the tauopathies and the selective vulnerability of different subsets of neurons. We demonstrate their usefulness and importance as tools for the dissection and comparative analysis of tau isoform expression and distribution as well as pathological changes in tauopathy brains using Western blots, immuno- histochemistry and cytochemistry and electron microscopy.
Supported by PSP (Eur) Assoc, Brain Res, Reta Lila Weston & Wellcome Trusts
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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