Neuroscience 2003 Abstract
| Presentation Number: | 504.3 |
|---|---|
| Abstract Title: | Differential CO-expression of various mRNA populations in single ventromedial hypothalamic neurons. |
| Authors: |
Devidze, N.*1
; Mong, J. A.1
; Kow, L. M.1
; Pfaff, D. W.1
1Dept. Neurobiol & Behavior, Rockefeller Univ, New York, NY |
| Primary Theme and Topics |
Autonomic, Neuroendocrine and Other Homeostatic Systems - Neuroendocrine -- Cellular signaling |
| Secondary Theme and Topics | Autonomic, Neuroendocrine and Other Homeostatic Systems<br />- Neuroendocrine<br />-- Steroids and plasticity |
| Session: |
504. Neuroendocrine: Cellular Signaling--Estrogen Poster |
| Presentation Time: | Monday, November 10, 2003 3:00 PM-4:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # I97 |
| Keywords: | ESTROGEN, OXYTOCIN, PKC, GENE EXPRESSION |
Studies with various neuronal and breast cancer cell lines show that activation of Protein Kinase C (PKC) is involved in the potentiating effect of the rapid membrane actions of estrogens (SFN Abst.’02 #482.6). Estrogens’ acute action can also increase the excitation of Ventromedial Hypothalamic (VMH) neurons and thereby facilitate their genomic action and effects on rats lordosis behavior (SFN Abst.’02 #482.6). There is high degree of interaction of estrogens and oxytocin in mice (Choleris et all. PNAS’ 03). Further to investigate possible co-expression of action of estrogen receptors, oxytocin receptors (OTR) and different isozymes of PKCs in single VMH neurons, RT-PCR was performed. Neurons were collected from 300-μm VMH slices of 3-5 week old, female and male rats. mRNA was reverse transcribed into cDNA, followed by real time quantitative PCR (ABI PRISM 7700). Primers were designed for the following receptors: ERα, ERβ, OTR, PKCα, PKCδ, PKCε, PKCζ (n=4 rats and 33 cells per group). OTR mRNA was co-expressed in 91% of female cells expressing ERα, while in males it was only 61%. Conversely ERα co-expression in OTR positive cells was 91% in female and 61% in males. For ERβ mRNA cells, 38% OTR was co-expressed in females and 67% in males. ERβ was co-expressed in 60 % of all ERα positive cells in females and in 79% of male cells. Only 41% of ERβ positive cells co-expressed ERα in females and 61% in males. In the PKC positive cells significant sex differences were found in PKCδ/ERα and PKCε/ERα co-expression; in male VMH co-expression of ERα was 1.8 and 2.2 times higher then in females respectively. Difference in ERβ mRNA co-expression was only in PKCζ positive cells where it was co-expressed 2.3 higher in males than in females. ER/OTR co-expression is in line with studies showing ER/OT and ER/PKC interactions in regulating rodent lordosis and social behaviors.
Supported by NIMH 38273 to D.W.P.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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