Neuroscience 2004 Abstract
| Presentation Number: | 518.10 |
|---|---|
| Abstract Title: | Glia involvement in central sensitization in trigeminal subnucleus caudalis (medullary dorsal horn). |
| Authors: |
Chiang, C. Y.*1
; Zhang, S.1
; Xie, Y. F.1
; Hu, J. W.1
; Dostrovsky, J. O.2
; Sessle, B. J.1,2
1Dept Physiol, Univ Toronto Fac Dent., Toronto, Canada 2ON, 124 Edward St, M5G 1G6, |
| Primary Theme and Topics |
Sensory Systems - Pain -- Trigeminal processing |
| Secondary Theme and Topics | Sensory Systems<br />- Pain<br />-- Spinal cord processing: phamacology |
| Session: |
518. Neuro-Glial Immune Interactions Poster |
| Presentation Time: | Monday, October 25, 2004 2:00 PM-3:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # O23 |
| Keywords: | ATP, NEUROPLASTICITY, INFLAMMATION, SODIUM FLUOROACETATE |
We have demonstrated that purinergic and NMDA receptor mechanisms are involved in the mustard oil (MO)-induced neuroplastic changes reflecting central sensitization in nociceptive neurons in trigeminal subnuclei caudalis (Vc; medullary dorsal horn) and oralis (Chiang et al. SfN Abst 175.14, 2003; Hu et al. J Neurophysiol 88:1614, 2002). This study tested whether Vc central sensitization induced by MO application to the tooth pulp depends on the functional integrity of glia by the use of a glia Kreb cycle blocker, sodium fluoroacetate (FA). Extracellular recordings of single neuronal activity were made from nociceptive-specific and wide dynamic range neurons in the deep Vc laminae in urethane/alpha-chloralose-anesthetized adult rats. The mechanoreceptive field (RF), mechanical activation threshold and responses to graded pinch or pressure of each neuron were assessed as baseline values. Then 2 assessments were made at 5 and 15 min after superfusion (i.t., 0.6 ml/hr for 50 min) of FA (1-10 mM) or saline (as control) onto Vc, and then MO was applied to the maxillary first molar tooth pulp and 5 assessments at 10 min intervals were begun 3 min after MO application. After saline pretreatment, MO application produced prolonged significant increases in neuronal RF size and responses to graded pinch or pressure and a significant decrease in threshold (all P<0.001, RM ANOVA, n=6). FA pretreatment significantly attenuated the MO-induced neuroplastic changes in 8 neurons (P>0.1-0.3, RM ANOVA), although FA itself produced an early and temporary (<30 min) increase in pinch-evoked responses and a decrease in threshold in 3 of these neurons. These results indicate that glia may play an important role in the initiation of central sensitization in nociceptive neurons located in the deep laminae of Vc.
Supported by NIH DE-04786
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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