Neuroscience 2002 Abstract
| Presentation Number: | 580.6 |
|---|---|
| Abstract Title: | STUDIES ON BLOOD-BRAIN BARRIER PERMEABILITY IN ACUTE STROKE IN RATS USING Gd-DTPA ENHANCED MRI AND ITS CONFIRMATION BY QUANTITATIVE AUTORADIOGRAPHY. |
| Authors: |
Nagaraja, T. N.*1
; Knight, R. A.2
; Nagesh, V.2
; Ewing, J. R.2
; Arniego, P.2
; Yee, J.2
; Randall, K. A.1
; Fagan, S. C.3
; Fenstermacher, J.1
1Anesthesiology, Henry Ford Health Sciences Center, Detroit, MI 2Neurology Research, Henry Ford Health Sciences Center, Detroit, MI 3College of Pharmacy, University of Georgia, Augusta, GA |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia -- Cellular and molecular mechanisms |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, tracing and imaging techniques |
| Session: |
580. Brain metabolism, transport and blood flow III Poster |
| Presentation Time: | Tuesday, November 5, 2002 2:00 PM-3:00 PM |
| Location: | Hall A2-B3 P-22 |
| Keywords: | Gd-DTPA, Imaging, Ischemia |
14C-labeled α-aminoisobutyric acid (AIB)-based quantitative autoradiography (QAR) was used to confirm MRI observations of loss of BBB integrity during reperfusion in a rat model of transient, unilateral, focal cerebral ischemia. All MRI observations were made using a 7-Tesla system. Diffusion- and T2-weighted imaging (DWI and T2WI, respectively) were performed during and after 3 hours of ischemia to identify the ischemic lesion. Qualitative and quantitative assessment of BBB integrity were done by serial scanning using T1-weighted imaging (T1WI) Look-Locker sequence to track a bolus injection of a gadolinium-chelate (Gd-DTPA). Immediately afterwards the rats were injected with a 14C-AIB bolus for QAR. The MRI images were processed to generate tissue segmentation maps and regions of interest (ROI) that demonstrated Gd-DTPA leakage. The ROI corresponded closely with regions showing 14C-AIB extravasation (i.e. BBB opening) on the autoradiograms. Transfer constants (Ki) were generated for the ROI from QAR data by conventional methods and from the slope of the time-dependent concentration curve of Gd-DTPA for MRI data. The Ki values from MRI and QAR were comparable. Similar comparative approaches validating MRI observations by established techniques such as QAR and quantitative morphometry may help to enhance the predictive powers and quantifying capabilities of MRI.
Supported by RO1 NS38540 and American Heart Association-Bugher Foundation Award.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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