Neuroscience 2004 Abstract
| Presentation Number: | 478.6 |
|---|---|
| Abstract Title: | Detection of neurotransmitter-induced neuronal activity using pharmacological activity-induced manganese-dependent contrast MRI. |
| Authors: |
Aoki, I.*1,2
; Lin Wu, Y.3
; Mori, Y.4
; Higuchi, T.4
; Ebisu, T.4
; Koretsky, A. P.2
; Tanaka, C.4
1Dept. of Med. Informatics, Meiji Univ. of Oriental Med., Kyoto, Japan 2MD, Kyoto, 629-0392, 3USA, Kyoto, 629-0392, 4Lab. of Functional and Mol. Imaging, Kyoto, 629-0392, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Session: |
478. Imaging Techniques: MRI, fMRI, and PET Slide |
| Presentation Time: | Monday, October 25, 2004 2:15 PM-2:30 PM |
| Location: | San Diego Convention Center - Room 2 |
| Keywords: | NEUROTRANSMITTER, MRI, ACTIVATION, BRAIN IMAGING |
Functional MRI (fMRI) based on blood oxygenation level dependent (BOLD) contrast relies on changes in brain hemodynamics. Recently, a new method for fMRI, named Activity-Induced Manganese Dependent (AIM) MRI, was reported as being independent of hemodynamics. We investigated whether AIM MRI would detect neurotransmitter-induced brain activity in the rat. The aim of this study is to produce new neurotransmitter-sensitive MR contrast. Dopamine (n = 5) and norepinephrine (n = 5) groups were used. The paradigm consisted of 6 steps as follows; 1) T1 weighted (T1W) MRI acquisition without manganese chloride (MnCl2), 2) 25% mannitol injection for BBB disruption, 3) MRI acquisition after MnCl2 and saline injection, 4) same acquisition as #3, 5) MRI acquisition after MnCl2 and neurotransmitter injection, and 6) MRI acquisition after MnCl2 and glutamate injection. There were 3 major results: 1) Neurotransmitter-induced brain activity mapping was successfully performed with phAIM MRI. 2) Dopamine-induced enhancement was observed in the hypothalamus (HT) and substantia nigra. 3) Norepinephrine-induced enhancement was observed in the HT (Fig.). These results indicate that phAIM MRI provides a new brain mapping tool reflecting pharmacological induced neural activity.
Supported by KAKENHI
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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