Neuroscience 2004 Abstract
| Presentation Number: | 478.2 |
|---|---|
| Abstract Title: | Using Mn-enhanced MRI <I>in vivo</I> neuronal tract tracing to compare functioning visual pathways in rabbits allowed and deprived eye use. |
| Authors: |
Dugas, J. P.*1
; Ji, T.1
; Wyrwicz, A. M.1,2
1Ctr for Basic MR Res, Evanston, IL 2IL, 1033 Univ Place Suite 150 , 60201, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Secondary Theme and Topics | Sensory Systems<br />- Vision<br />-- Subcortical visual pathways |
| Session: |
478. Imaging Techniques: MRI, fMRI, and PET Slide |
| Presentation Time: | Monday, October 25, 2004 1:15 PM-1:30 PM |
| Location: | San Diego Convention Center - Room 2 |
| Keywords: |
Previous work has shown that Mn2+ enters neurons through voltage-gated Ca2+ channels and is transported along axons (anterograde transport) and across synapses during neuronal activation. Paramagnetic Mn2+ shortens the T1 relaxation time of water molecules in its immediate environment enabling visualization of neuronal tracts as hyper-intense regions in T1-weighted MR images. Results from our examination of visual pathways in use-allowed and use-deprived eyes in the rabbit, employing manganese-enhanced MRI to trace functioning neuronal connections, are presented. For our experiments, 1-5 μL of aqueous 1.0 M MnCl2 solution was injected into the vitreous body of one eye of Dutch-belted rabbits. T1-weighted, multi-slice, spin-echo MR images (TR=424 ms, TE=14.6 ms) were acquired on a Bruker Biospin 4.7 Tesla imager immediately following injection with subsequent imaging performed every 48 hours for up to 10 days. Consecutive, 1mm thick slices were acquired in the coronal, axial, and sagittal planes. Deprivation of eye-use in selected animals was accomplished through eyelid closure immediately following injection and maintained throughout the 10-day imaging period. We measured MR signal intensity along the optical tract along with the time of Mn2+ uptake and transport to ascertain any differences in the pathway activated. Crossover of the agent to the contralateral side of the brain was observed in the use-allowed eye, followed by transport of the agent into the lateral geniculate nucleus, superior colliculus, and possibly visual cortex.
Supported by NIH under grant MH58912
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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