Neuroscience 2004 Abstract
| Presentation Number: | 478.1 |
|---|---|
| Abstract Title: | A MRI-compatible stereotaxic localizer box enables high-precision stereotaxic procedures in pigs. |
| Authors: |
Bjarkam, C. R.*1
; Cancian, G.2
; Larsen, M.1
; Rosendahl, F.1
; Ettrup, K. S.1
; Zeidler, D.
; Blankholm, A. D.
; Østergaard, L.
; Sunde, N. A.
; Sørensen, J.1
1Dept Neurobiol, Univ Aarhus, Aarhus, Denmark 2Denmark, Inst Anatomy, DK-8000, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Session: |
478. Imaging Techniques: MRI, fMRI, and PET Slide |
| Presentation Time: | Monday, October 25, 2004 1:00 PM-1:15 PM |
| Location: | San Diego Convention Center - Room 2 |
| Keywords: | BASAL GANGLIA, ELECTROPHYSIOLOGY, STEM CELL, TRANSPLANTATION |
Introduction: We present a nonmagnetic Plexiglas stereotaxic localizer box that can be fitted directly to the pig skull by aluminum screws, allowing stereotaxic MRI or ventriculography and subsequent high-precision stereotaxic procedures.
Methods: The localizer box was used to target the subthalamic nucleus (STN) bilaterally in 5 female Göttingen minipigs. Stereotaxic markers were inserted in the pig skull, the head fixated in the localizer box by aluminum screws inserted bilaterally in the zygoma bone with the hard palate locked on a horizontal palate holder. MRI was obtained on a 3T-MR-imager revealing the relation between the inserted markers and the estimated STN-position, and thus the target coordinates. After the MRI a stereotaxic frame with attached micromanipulator was locked on to the localizer box converting it into a stereotaxic device. The stereotaxic markers were exposed and used as starting point for the stereotaxic procedure, whereby a microelectrode for electrolytic lesioning was inserted in the STN.
Results: Postmortem histological analysis revealed 70% correct STN-targeting. The average distance from the lesion center to the STN center was 1.2 mm with a SD of 1.1 mm. The most displaced lesion being 3.6 mm from the STN center.
Conclusion: The described localizer box secure firm head fixation, allowing stereotaxic MRI and subsequent conversion into a stereotaxic device for high-precision stereotaxic procedures.
Methods: The localizer box was used to target the subthalamic nucleus (STN) bilaterally in 5 female Göttingen minipigs. Stereotaxic markers were inserted in the pig skull, the head fixated in the localizer box by aluminum screws inserted bilaterally in the zygoma bone with the hard palate locked on a horizontal palate holder. MRI was obtained on a 3T-MR-imager revealing the relation between the inserted markers and the estimated STN-position, and thus the target coordinates. After the MRI a stereotaxic frame with attached micromanipulator was locked on to the localizer box converting it into a stereotaxic device. The stereotaxic markers were exposed and used as starting point for the stereotaxic procedure, whereby a microelectrode for electrolytic lesioning was inserted in the STN.
Results: Postmortem histological analysis revealed 70% correct STN-targeting. The average distance from the lesion center to the STN center was 1.2 mm with a SD of 1.1 mm. The most displaced lesion being 3.6 mm from the STN center.
Conclusion: The described localizer box secure firm head fixation, allowing stereotaxic MRI and subsequent conversion into a stereotaxic device for high-precision stereotaxic procedures.
Supported by HistOtech ApS
<B>Conflict of Interest:</B> The study was in part financially supported by HistOtech ApS.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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