Neuroscience 2003 Abstract
| Presentation Number: | 462.7 |
|---|---|
| Abstract Title: | Functional localization of endogenous cannabinoid production in distinct neuron populations of the hippocampus. |
| Authors: |
Lupica, C. R.*1
; Riegel, A. C.1
; Hoffman, A. F.1
1Cell. Neurophysiol. Unit, NIDA-IRP, NIH/DHHS, Baltimore, MD |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Neurotransmitters -- Cannabinoids |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Synaptic Transmission<br />-- Presynaptic mechanisms |
| Session: |
462. Cannabinoids I Poster |
| Presentation Time: | Monday, November 10, 2003 3:00 PM-4:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # C22 |
| Keywords: | INTERNEURON, DRUG ABUSE, ELECTROPHYSIOLOGY, GABA |
Activation of cannabinoid receptors by the active ingredients of marijuana disrupt memory via actions in the hippocampus. We have shown that activation of cannabinoid CB1 receptors by the synthetic agonist WIN55,212-2 inhibits GABAergic IPSCs in hippocampal pyramidal cells and interneurons, implying that axon terminals impinging on both cell types possess these receptors. In the present study, we compared the involvement of endogenous cannabinoids (eCBs) in the presynaptic inhibition of GABA release in interneurons and pyramidal cells by measuring a response known to be dependent upon eCBs (depolarization-induced suppression of inhibition, DSI). Interneurons and pyramidal cells were recorded in hippocampal slices using DIC-IR microscopy. DSI was observed in 50% of pyramidal neurons under control conditions, and its incidence was greatly increased (90%) by the cholinergic agonist carbachol (CCh). However, DSI was not observed in interneurons located in strata radiatum (SR) or oriens (SO), in the presence or absence of CCh. Whereas DSI was not present in the interneurons, IPSCs in these cells were inhibited by WIN55,212-2. These data support the hypothesis that pyramidal neurons, and not interneurons, are capable of generating eCBs during depolarization.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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