Neuroscience 2005 Abstract
| Presentation Number: | 489.12 |
|---|---|
| Abstract Title: | Lysergic acid diethylamide-induced BOLD signal in rat brain using functional magnetic resonance imaging. |
| Authors: |
Gresch, P. J.*1
; Turner, G. H.4
; Hackler, E. A.1
; Avison, M. J.1,3,4
; Gore, J. C.3,4
; Sanders-Bush, E.1,2
1Pharmacology, Vanderbilt Univ. School of Medicine, Nashville, TN 2Psychiatry, Vanderbilt Univ. School of Medicine, Nashville, TN 3Radiology and Radiological Sciences, Vanderbilt Univ. School of Medicine, Nashville, TN 4Inst. of Imaging Science, Vanderbilt Univ. School of Medicine, Nashville, TN |
| Primary Theme and Topics |
Neural Excitability, Synapses, and Glia: Cellular Mechanisms - G-Protein Linked Receptors -- Serotonin receptors |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Addiction and Drugs of Abuse<br />-- Hallucinogens |
| Session: |
489. Serotonin Receptors II Poster |
| Presentation Time: | Monday, November 14, 2005 4:00 PM-5:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # D54 |
| Keywords: | serotonin, MRI, hallucinogen, anterior cingulate cortex |
Lysergic acid diethylamide (LSD) is a potent drug that produces distorted perceptions, disorganized thought, and altered mood in humans. At present, the neuronal substrates mediating these effects are poorly understood. In this study, we used functional magnetic resonance imaging (fMRI) to evaluate LSD-induced blood oxygen level dependent (BOLD) response in rat brain. Briefly, rats (n=3) were anesthetized with isoflurane (1%), paralyzed with pancuronium (0.5 mg/kg, i.p.), artificially ventilated (50 breaths/min, tidal volume of 3 ml), and placed in a Varian 9.4 Tesla MRI instrument. After collecting basal volume data sets for 20 min, rats received LSD (500 µg/kg, i.p.) and volume data sets were collected for 40 min post drug. Mean voxel values averaged across a 5 min period prior to and 25-30 min after LSD administration were compared using Student's t-test (significance level set at p < 0.001). Anatomical localization of the BOLD signal was determined by using a rat brain atlas template (Paxinos and Watson, 1986) as reference. Significant increases in BOLD signal were detected in frontal cortex, parietal motor cortex, anterior striatum, lateral septum, nucleus accumbens, diagonal band of broca, claustrum, deep layers of anterior cingulate cortex, and somatosensory cortex. Significant decreases in BOLD signal were measured in superficial layers of anterior cingulate cortex, somatosensory cortex, mid-dorsal striatum, dorsal lateral septum, hippocampus, lateral thalamus, and central medial thalamus. These results indicate that fMRI will be a powerful tool to determine brain activation patterns, neuronal circuitry, and pharmacology of hallucinogen drug response in laboratory animals.
Supported by DA05181, EB002326
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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