Neuroscience 2000 Abstract
| Presentation Number: | 505.21 |
|---|---|
| Abstract Title: | Transferrin effects on the expression of myelin basic protein (MBP) after sciatic nerve crush. |
| Authors: |
Setton-Avruj, C. P.*1
; Aquino, J.2
; Goedelmann, C.1
; Pasquini, J. M.1
; Soto, E. F.1
; Villar, M. J.2
1Department of Biochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires - IQUIFIB-CONICET, 2Faculty of Biomedical Sciences, Austral University, Buenos Aires, Argentina |
| Primary Theme and Topics |
A. Development and Regeneration - 4. Glial and other non-neuronal cell specification and differentiation |
| Secondary Theme and Topics | <br />- 22. Regeneration |
| Session: |
505. Glial and other non-neuronal cell specification and differentiation II Poster |
| Presentation Time: | Tuesday, November 7, 2000 1:00 PM-2:00 PM |
| Location: | Hall G-J |
| Keywords: | Nerve Crush, Nerve Degeneration, Demyelination, Myelin Proteins |
Myelin basic proteins (MBPs), are major proteins which account for about 30% of total myelin protein content in peripheral nervous system. In several reports some MBPs have been related to mechanisms of nerve regeneration and nerve ligation was shown to alter differentially the expression of MBP from that of P0 in young and adult rats (Setton-Avruj et al. SFN 1999). In this work we have analyzed variations in the expression of MBPs in the sciatic nerve after crush and injection of apotransferrin (Tf). Animals were anaesthetized and their right sciatic nerves exposed at the level of the tigh and injected with 6 μl of Tf. Immediately after the injection, the nerve was crushed proximally to the injection site during 3 sec. Animals were allowed to survive for 1, 3, 7 and 14 days. Sham animals with crushed nerves and saline injections were also prepared. The animals were perfused and the ipsilateral and contralateral sciatic nerves were processed with antibodies against MBPs. The ipsilateral nerve was divided in two regions, one close to the crush and another one 20 mm distant from the crush. The results showed a normal pattern of MBPs-like immunoreactivity (LI) in contralateral sciatic nerves. In sham operated animals clusters of MBPs-LI were detected distally to the crush. These clusters were observed after one day of survival reaching a peak at three days in the zone close to the crush. More distally the higher number of clusters was observed at 14 days. Interestingly, with Tf injection a significantly lower number of clusters with MBP-LI was observed in both regions of the nerve. The functional significance of the clusters displaying MBPs-LI has been interpreted as a sign of degradation of the lesioned sciatic nerve. Whether or not the lower numbers of these clusters in Tf injected animals is a sign of protection of the injured nerve by blockage of normal degradative mechanisms after lesion is currently under study.
Supported by <I>CONICET and Buenos Aires and Austral Universities.</I>
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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