Neuroscience 2005 Abstract
| Presentation Number: | 458.10 |
|---|---|
| Abstract Title: | Imagesurfer reloaded: interactive 3D analysis of multiple-channel confocal images. |
| Authors: |
Marshburn, D.*1
; Taylor II, R. M.1
; Weinberg, R. J.2,3
; Burette, A.3
1Computer Science, Univ. of North Carolina at Chapel Hill, Chapel Hill, NC 2Neuroscience Center, Univ. of North Carolina at Chapel Hill, Chapel Hill, NC 3Cell & Developmental Biology, Univ. of North Carolina at Chapel Hill, Chapel Hill, NC |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Data Analysis and Statistics |
| Session: |
458. Physiological, Morphological, and Culture Methods Poster |
| Presentation Time: | Monday, November 14, 2005 9:00 AM-10:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # WW35 |
| Keywords: | IMMUNOFLUORESCENCE, MICROSCOPY, SOFTWARE, VOLUME RENDERING |
Confocal microscopy, a key tool in neuroscience, is designed to provide 3D information. However, in practice, analysis of multiple labeling is almost exclusively performed on 2D images, reflecting the lack of effective tools for analyzing multi-channel images in 3D. For this reason we are developing a new software application, ImageSurfer, an initial version of which was reported at the 2004 SFN meeting. ImageSurfer is an interactive software package for volume visualization and data analysis that enables researchers to quickly explore complex 3D confocal images. It is designed to be user-friendly and intuitive with a gentle learning curve. ImageSurfer incorporates traditional visualization tools such as maximum intensity projection, direct volume rendering, and isosurface rendering; and advanced tools to analyze and quantify relationships between multi-channel confocal images. A specialized colored isosurface technique permits display of an easily-understood 3D view showing the correspondence between two channels. Detailed analysis can be carried out using a 2D slice extractor combined with display of height fields; data can be extracted along a user-defined curve and exported to standard statistical software. This 2005 version of ImageSurfer makes use of Java classes from ImageJ (the widely-used public domain program developed by Wayne Rasband at NIH). This allows ImageSurfer to read the most common file formats including DICOM and Leica format, and enhanced data exploration and analysis through new tools.
ImageSurfer is built, using Java, on top of the VTK visualization toolkit. It can run on standard laboratory computers with modest processor speeds and memory size. A free download for Microsoft Windows, along with documentation, is available at www.ImageSurfer.org.
ImageSurfer is built, using Java, on top of the VTK visualization toolkit. It can run on standard laboratory computers with modest processor speeds and memory size. A free download for Microsoft Windows, along with documentation, is available at www.ImageSurfer.org.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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