Neuroscience 2005 Abstract
| Presentation Number: | 456.18 |
|---|---|
| Abstract Title: | Local gadolinium-microinjections for MRI documentation of microelectrode recording or injection sites. |
| Authors: |
Linn, J.*1
; Kleine, J. F.2
; Langer, S.2
; Tang, X.2
; Gehr, D.2
; Wiesmann, M.1
; Buttner-Ennever, J.3
; Buttner, U.2
1Neuroradiology, LMU Muenchen, Muenchen, Germany 2Neurology, LMU Muenchen, Muenchen, Germany 3Anatomy, LMU Muenchen, Muenchen, Germany |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Session: |
456. Imaging by MRI and PET II Poster |
| Presentation Time: | Monday, November 14, 2005 9:00 AM-10:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # VV86 |
| Keywords: |
Microelectrode recording and stimulation techniques as well as microinjections of pharmacologic agents to record or manipulate the activity of neuronal cell groups play an outstanding role in neurophysiology. Commonly, such experiments depend on accurate determination of the anatomic structure investigated. This has traditionally been done histologically by placing small dye marks or electrolytic lesions which are visualised on brain sections after sacrificing the experimental animal. MRI is now widely used as an aid in the planning of neurophysiological experiments to improve stereotaxic localisation of target structures. To further the benefit from MRI we developed a technique similar to traditional histological procedures, to directly visualise recording sites in deep brain structures of the monkey, consisting in local injections of small volumes of gadolinium (GDA), a clinically used MRI contrast agent. These GDA-microinjections were visualized by MRI sequences [TIR (TR=3500 ms, TE=66 ms, TI=350 ms) and MPRAGE (TR=1280 ms, TE=4.39 ms, voxel size 0.8 mm³)] on a clinical 1.5 T scanner (Siemens Symphony), performed in the anesthetised monkey. The microinjections appeared as spherical marks of 1-2 voxel diameter that could be detected for at least 60 to 90 minutes after injection. After recovery from anesthesia, monkeys showed no signs of neurological dysfunction nor other toxic side effects. To compare the performance of the technique with conventional histology, we matched MRI scans and brain sections of the same animal after simultaneous injection of GDA and a tract tracer substance (tetramethyl rhodamine). Histological examination of GDA-injection sites did not reveal evidence of toxicity or local tissue damage. Local GDA-microinjections thus seem to be a safe and valuable procedure to document recording or injection sites that can be performed repeatedly in the same animal and may often suffice to replace conventional histology.
Supported by DFG, EU
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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