Neuroscience 2005 Abstract
| Presentation Number: | 456.13 |
|---|---|
| Abstract Title: | <i>In vivo</i> magnetic resonance imaging of grafted neural stem cells in the spinal cord injury. |
| Authors: |
Ishii, K.*1
; Fujiyoshi, K.1,2
; Ikegami, T.1,2
; Okada, S.2
; Yamada, M.3
; Okano, H. J.2
; Nakamura, M.1
; Okano, H.2
; Toyama, Y.1
1Dept Orthopaedic Surgery, Keio Univ. School of Medicine, Tokyo, Japan 2Dept Physiology, Keio Univ. School of Medicine, Tokyo, Japan 3Central Inst. for Experimental Animals, Keio Univ. School of Medicine, Tokyo, Japan |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Trauma<br />-- Spinal cord: Cellular and molecular mechanisms |
| Session: |
456. Imaging by MRI and PET II Poster |
| Presentation Time: | Monday, November 14, 2005 8:00 AM-9:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # VV86 |
| Keywords: | <i>in vivo</i> imaging, MRI, neural stem cells, spinal cord injury |
Real-time in vivo imaging of grafted neural stem progenitor cells (NSPCs) is a significant advance toward understanding the fate of NSPCs after grafting. We have previously demonstrated the utility of bioluminescence imaging system for tracking grafted primary NSPCs in the injured spinal cord. Here we investigated the use of magnetic resonance imaging (MRI) to track the contrast agent-labeled primary NSPCs in the injured spinal cord. Primary NSPCs derived from E14 rodent striatum were labeled with iron oxides particles. Magnetically labeled NSPCs exhibited a similar growth rate to unlabeled cells and did not affect normal cellular differentiation. MRI was performed on an either 11.7-T Bruker Advance Spectrometer or 7.0-T Bruker Pharmascan. in vitro MRI showed that one cluster consisting of 200 single cells led to an MRI contrast sufficient to allow reliable detection. in vivo experiment, spinal cord injury was induced by Multicenter Animal Spinal Cord Injury Study (MASCIS) impactor at T10 level and NSPCs were transplanted into lesion epicenter. Magnetically labeled NSPCs could be readily detected at least as long as 4 weeks after transplantation. There was an excellent correspondence between histological and in vivo MR imaging with respect to the location of NSPCs.
We have demonstrated that magnetically labeled primary NSPCs can be easily monitored at the cellular level in vivo and this technology may be directly applied as a non-invasive measurement of grafted NSPCs in a clinical setting.
We have demonstrated that magnetically labeled primary NSPCs can be easily monitored at the cellular level in vivo and this technology may be directly applied as a non-invasive measurement of grafted NSPCs in a clinical setting.
Supported by Keio Gijuku Academic Development Funds, National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University, and Uehara Memorial Foundation
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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