Neuroscience 2002 Abstract
| Presentation Number: | 519.2 |
|---|---|
| Abstract Title: | IN VIVO AXOPLASMIC TRACING OF THE RAT OLFACTORY SYSTEM USING MAGNETIC RESONANCE IMAGING. |
| Authors: |
Cross, D. J.*1,2
; Anzai, Y.1
; Keogh, B. P.1
; Maravilla, K. R.1
; Shankland, E.1
; Minoshima, S.1
1Dept. of Radiology, University of Washington, Seattle, WA 2Neuroscience Program, University of Michigan, Ann Arbor, MI |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Secondary Theme and Topics | Sensory Systems<br />- Chemical Senses<br />-- Olfaction: CNS pathways and physiology |
| Session: |
519. Staining, tracing, and imaging techniques VI Slide |
| Presentation Time: | Tuesday, November 5, 2002 1:15 PM-1:30 PM |
| Location: | Room 307A |
| Keywords: | MRI, OLFACTORY, TRANSPORT, IN VIVO |
The olfactory system is a unique route for uptake and transport of materials to the central nervous system. The purpose of this study is to trace transport of a paramagnetic substance, manganese, in the rat olfactory system using serial magnetic resonance imaging. Sequential magnetic resonance imaging on a 1.5T MR scanner using a rat brain volume coil and 3D SPGR pulse sequence was performed at pre-injection, and 6, 9, 12, 24, 36, and 48 hour post injection of 10 microliter MnCl2 solution via polyethylene catheter into the right nasal cavity in 5 male Sprague Dawley rats under isoflurane anesthesia. Changes in signal intensity were measured by region-of-interest analysis as well as voxel-by-voxel subtraction analysis of post-pre injection images using image coregistration. Signal intensity changes of greater than 1.9 target to muscle ratio were seen progressively in the right olfactory epithelium (post injection), olfactory bulb (< 6 hrs), olfactory tubercle (<9 hrs), piriform cortex (<9 hrs), and entorhinal cortex (<24 hrs). Image subtraction analysis demonstrated trans-synaptic transport of manganese through anterior commissure and anterior olfactory nucleus. Rate of manganese transport from the olfactory epithelium to the cortex was approximately 1.3 mm/hr. This study illustrates progressive trans-synaptic transport of manganese in the rat olfactory system in a non-invasive and repeatable manner. The technique can be applied to anatomic and physiologic research of the olfactory system and has potential applications to monitor intranasal drug delivery. Supported by the University of Washington ADRC.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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