Neuroscience 2005 Abstract
| Presentation Number: | 456.6 |
|---|---|
| Abstract Title: | Manganese-enhanced MRI of normal and lesioned optic nerve in adult frog and fish. |
| Authors: |
Sandvig, A.*1
; Brekken, C.2
; Thuen, M.2
; Sandvig, I.1
; Pedersen, T. B.2
; Haraldseth, O.2
; Berry, M.3
1Laboratory of Regenerative Neurobiology, Inst. for Experimental Medical Research, Ulleval Univ. Hospital, Univ. of Oslo, Oslo, Norway 2Norway, 4th floor surgical building,, 0407, 3FUGE Molecular Imaging Center, Department of Circulation and Medical Imaging, 4th floor surgical building,, 0407, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Session: |
456. Imaging by MRI and PET II Poster |
| Presentation Time: | Monday, November 14, 2005 9:00 AM-10:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # VV86 |
| Keywords: | MRI, VISUAL, NERVE INJURY, AXONAL TRANSPORT |
Introduction: The use of manganese chloride as a contrast agent for visualizing specific axonal projections with MRI is currently a topic under intense investigation. We demonstrate here the use of this technique to visualize normal and lesioned optic nerve in adult frog and fish.
Materials and Methods: Adult frogs (rana pipiens, n=5) and adult fish (brown trout, n=5) were subjected to unilateral optic nerve transection followed by ipsilateral intravitreal injection of 2 ul of 100 mM manganese chloride. Normal control animals (n=3) underwent unilateral intravitreal injections of 2 ul of 100 mM of manganese chloride. All procedures were in accordance with national and local authority regulations. MRI was performed at 2.35 T using a Bruker Biospec Avance DBX-100 (Bruker AG, Germany) in accordance with a modified protocol by Brekken et al. 2003.
Results: MRI performed at 48-72 hours post-injection demonstrated specific contrast uptake and transport in vivo throughout the visual system of both frog and fish. Furthermore, after optic nerve transection, the manganese-enhanced MRI signal was present proximal to the nerve injury, but not distal to the lesion site.
Conclusion: We have demonstrated the potential of manganese chloride as a MRI contrast agent to specifically visualize normal and lesioned optic nerve in adult frog and fish. This technique offers the potential to study nerve regeneration after CNS damage in regenerative animal models.
References:
Brekken, C. et al.: Abstract. ISMRM workshop on cellular and molecular imaging, Bordeaux. France. June 29- July 1 2003.
Materials and Methods: Adult frogs (rana pipiens, n=5) and adult fish (brown trout, n=5) were subjected to unilateral optic nerve transection followed by ipsilateral intravitreal injection of 2 ul of 100 mM manganese chloride. Normal control animals (n=3) underwent unilateral intravitreal injections of 2 ul of 100 mM of manganese chloride. All procedures were in accordance with national and local authority regulations. MRI was performed at 2.35 T using a Bruker Biospec Avance DBX-100 (Bruker AG, Germany) in accordance with a modified protocol by Brekken et al. 2003.
Results: MRI performed at 48-72 hours post-injection demonstrated specific contrast uptake and transport in vivo throughout the visual system of both frog and fish. Furthermore, after optic nerve transection, the manganese-enhanced MRI signal was present proximal to the nerve injury, but not distal to the lesion site.
Conclusion: We have demonstrated the potential of manganese chloride as a MRI contrast agent to specifically visualize normal and lesioned optic nerve in adult frog and fish. This technique offers the potential to study nerve regeneration after CNS damage in regenerative animal models.
References:
Brekken, C. et al.: Abstract. ISMRM workshop on cellular and molecular imaging, Bordeaux. France. June 29- July 1 2003.
Supported by FUS, Ulleval University Hospital, Oslo, Norway
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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