Neuroscience 2005 Abstract
| Presentation Number: | 454.7 |
|---|---|
| Abstract Title: | An MRI correlate of dentate gyrus neurogenesis in mice. |
| Authors: |
Huddleston, D.*1
; Sosunov, A.1
; Kelm, M.3
; Hammerstone, J.3
; McKhann, G.1
; Gage, F. H.2
; Small, S. A.1
1Columbia Univ., New York, NY 2CA, 630 West 168 St., 10032, 3USA, 630 West 168 St., 10032, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, Tracing, and Imaging Techniques |
| Session: |
454. Imaging by MRI and PET I Poster |
| Presentation Time: | Monday, November 14, 2005 10:00 AM-11:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # VV46 |
| Keywords: | BRDU, BRAIN IMAGING, EXERCISE |
Introduction: Currently, detecting neurogenesis in the dentate gyrus can only be accomplished in post-mortem tissue. Imaging neurogenesis in living subjects is needed in order to establish its functional significance. Neurogenesis is coupled to angiogenesis, which in turn is coupled to cerebral blood volume (CBV), a variable that can be measured with MRI. In principle, dentate gyrus CBV measured with MRI is expected to be sensitive to neurogenesis. Nevertheless, any manipulation that induces neurogenesis will also cause non-neurogenesis effects, which may also affect CBV. Thus, dentate gyrus CBV is sensitive but not specific to neurogenesis. We hypothesized that we could impose specificity by adjusting dentate gyrus CBV with CBV measured from the CA1 subfield, a neighboring hippocampal subregion that reflects non-neurogenesis factors. Here, we test this hypothesis by determining whether adjusted dentate gyrus CBV, as measured in vivo with MRI, is correlated with neurogenesis.
Methods: We used MRI to measure CBV changes over time in hippocampal subregions of mice who were exposed to two stimulants of neurogenesis-- exercise and fluoxetine. Post-mortem, BRDU labeling was then used to estimate cell proliferation in the dentate gyrus of each mouse.
Results: As predicted, dentate gyrus CBV, adjusted for CA1 CBV, significantly correlated with the degree of cell proliferation induced by the manipulations.
Discussion: By relying on the spatial profile of CBV changes in multiple hippocampal subregions, we can, for the first time, predict the degree of cell proliferation in living subjects. We are currently extending these findings by staining cells with neuron specific markers.
Methods: We used MRI to measure CBV changes over time in hippocampal subregions of mice who were exposed to two stimulants of neurogenesis-- exercise and fluoxetine. Post-mortem, BRDU labeling was then used to estimate cell proliferation in the dentate gyrus of each mouse.
Results: As predicted, dentate gyrus CBV, adjusted for CA1 CBV, significantly correlated with the degree of cell proliferation induced by the manipulations.
Discussion: By relying on the spatial profile of CBV changes in multiple hippocampal subregions, we can, for the first time, predict the degree of cell proliferation in living subjects. We are currently extending these findings by staining cells with neuron specific markers.
Supported by DARPA
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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