Neuroscience 2001 Abstract
| Presentation Number: | 507.13 |
|---|---|
| Abstract Title: | FUNCTIONAL RESPONSE TO SENSORY STIMULATION MONITORED IN RAT AND MICE BY SPIRAL fMRI. |
| Authors: |
Schweinhardt, P.*1,2
; Fransson, P.2
; Klason, T.2
; M蚣son, S.3
; Olson, L.1
; Spenger, C.1,2
1Dept. of Neuroscience, Karolinska Institutet, Stockholm, Sweden 2Experimental Unit of MR Center, Karolinska Institutet, Stockholm, Sweden 3Dept. of Experimental Research, University Hospital, Malmö, Sweden |
| Primary Theme and Topics |
Sensory Systems - Pain -- Functional imaging, psychophysics, human behavior |
| Secondary Theme and Topics | Development<br />- Transplantation and Regeneration<br />-- Regeneration |
| Session: |
507. Pain: functional imaging Poster |
| Presentation Time: | Tuesday, November 13, 2001 8:00 AM-9:00 AM |
| Location: | Exhibit Hall R-8 |
| Keywords: | magnetic, spinal cord, pain, cortex |
Functional MRI based on the blood oxygenation level-dependent (BOLD) contrast in gradient echo images has been used to monitor cerebral activation in response to peripheral stimuli in rat. No previous report on functional MRI during peripheral stimulation is available for mice.
Functional MRI experiments were performed in rats and in mice using a 4.7-Tesla MRI scanner. Periodical electric stimulation (10-15 sec. stimulation, 40-60 sec. rest) was delivered at 3 Hz via bipolar subcutaneous electrodes during α-Chloralose anesthesia, artificial ventilation and complete muscle relaxation as reported earlier (Spenger et al., Exp Neurol 2000, 166:1102). Data were obtained in single and multislice experiments using spiral gradient echo MR imaging. We could thus improve the time resolution to 300 ms per image, which is approximately 13 times faster than conventional gradient echo imaging. In rats, the rise time for the BOLD response was found to be in the range of 4 sec. The BOLD signal was usually followed by an undershoot lasting for more than 20 sec. indicating the importance of long resting intervals for fMRI experiments.
In the mouse robust and contralateral activation of the somatosensory cortex and the thalamus was observed following hindpaw stimulation.
In sum valuable information on somatosensory connectivity can be obtained by fMRI in rodents including mice. Using spiral fMRI the time sequential behavior of the BOLD response can be described accurately.
Functional MRI experiments were performed in rats and in mice using a 4.7-Tesla MRI scanner. Periodical electric stimulation (10-15 sec. stimulation, 40-60 sec. rest) was delivered at 3 Hz via bipolar subcutaneous electrodes during α-Chloralose anesthesia, artificial ventilation and complete muscle relaxation as reported earlier (Spenger et al., Exp Neurol 2000, 166:1102). Data were obtained in single and multislice experiments using spiral gradient echo MR imaging. We could thus improve the time resolution to 300 ms per image, which is approximately 13 times faster than conventional gradient echo imaging. In rats, the rise time for the BOLD response was found to be in the range of 4 sec. The BOLD signal was usually followed by an undershoot lasting for more than 20 sec. indicating the importance of long resting intervals for fMRI experiments.
In the mouse robust and contralateral activation of the somatosensory cortex and the thalamus was observed following hindpaw stimulation.
In sum valuable information on somatosensory connectivity can be obtained by fMRI in rodents including mice. Using spiral fMRI the time sequential behavior of the BOLD response can be described accurately.
Supported by Swedish MFR, AMF, NIDA/NIH
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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