Neuroscience 2004 Abstract
| Presentation Number: | 430.19 |
|---|---|
| Abstract Title: | Mri characterisation of a novel rat model of focal astrocyte loss. |
| Authors: |
Brown, A. M.*1
; Prior, M. J. W.2
; Mavroudis, G.1
; Lister, T.1
; Ray, D. E.1
1Sch. of Biomed. Sci., Univ. of Nottingham, Nottingham, United Kingdom 2Sir Peter Mansfield Magnetic Resonance Centre, Univ. of Nottingham, Nottingham, United Kingdom |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Brain Blood Flow, Metabolism, and Homeostasis -- Blood brain barrier |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Neurotoxicity<br />-- Toxic metabolic effects and disorders |
| Session: |
430. Blood--Brain Barrier II Poster |
| Presentation Time: | Monday, October 25, 2004 10:00 AM-11:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # II8 |
| Keywords: | MRI, BLOOD-BRAIN BARRIER, ASTROCYTE |
Blood brain barrier (BBB) integrity is lost several neurological conditions in which astrocytes are damaged. We have studied the focal lesions induced by systemic injection of 3-choloropropanediol, a toxicant that induces early astrocytic (but not neuronal) death followed by BBB leakage. Anatomical images of coronal brain sections illustrate the regional selectivity of the lesions, with affected areas including symmetrical brain stem and midbrain nuclei: primarily the inferior colliculi and red nuclei. The MRI intensity in the lesioned areas peaked at 2 days post-injection, correlating with the later BBB breakdown, and diminished thereafter; returning to close to pre-injection levels by 30 days in parallel with the return of astrocytes. T2 measurements of the lesioned areas increased at 2 days indicating increased water content in the lesioned areas, consistent with cellular damage and BBB leakage. T2 was not increased at 6 hours, a time when astrocyte swelling is marked but the vasculature is intact. Injection of Gd-DTPA was used to image the leaky areas. Gd enhancement was also greatest at 2 days then decreased over the next 28 days, indicating a tracer size dependant rate of BBB repair. A simple model based on experimentally acquired data indicated that the 3-chloropropanediol-induced vascular breakdown was the result of leakage of only a small percentage of blood vessels in the affected areas. We conclude that loss of astrocytes contributes to barrier loss, and that restoration of astrocytes is needed for full barrier recovery.
Supported by MRC
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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