Neuroscience 2003 Abstract
| Presentation Number: | 414.6 |
|---|---|
| Abstract Title: | Effect of temperature on post-traumatic spontaneous epileptiform activity in the rat hippocampus. |
| Authors: |
Tian, G.*1,2
; Zhao, X.1,2
; Wang, J.1,2
; Baker, A. J.1,2
1Trauma Res., St. Michael's Hosp., Toronto, Canada 2Anaesthesia, St. Michael's Hosp., Toronto, Canada |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Trauma -- Brain |
| Session: |
414. Trauma: Brain II Poster |
| Presentation Time: | Monday, November 10, 2003 9:00 AM-10:00 AM |
| Location: | Morial Convention Center - Hall F-I, Board # LL3 |
| Keywords: | BRAIN, TRAUMA, HYPEREXCITABILITY, EPILEPSY |
Post-traumatic spontaneous epileptiform activities (SEAs) can be recorded in rat hippocampal slices at 36.5 ± 0.5 degrees Celsius (Tian et al., Program No.891.16, 2002 SfN Abstract). This novel observation at the rodents’ physiological temperature might be a phenomenon that only exists at such a relatively high temperature, whereas most investigators make recordings in hippocampal slices at lower temperatures (32 to 34 degrees). This study was designed to investigate whether post-traumatic SEAs can be recorded in rat hippocampal slices at a lower temperature and whether temperature affects post-traumatic SEAs. Anaesthetized adult male rats were subjected to moderate lateral fluid-percussion (F-P) brain injury. Horizontal hippocampal slices were
obtained from rats at 1-day post F-P brain injury. Extracellular recordings were performed in CA3c of slices from the hippocampus ipsilateral to the F-P brain injury. SEAs were recorded in 64 ± 15% (mean ± SD) of tested slices from 5 rats at 36.5 ± 0.5 degrees, and in 60 ± 14% of tested slices from 4 rats at 32.5 ± 0.5 degrees (P>0.05). Frequencies of SEAs were 0.08 ± 0.03 (n=16) and 0.07 ± 0.04 Hz (n=12) at 36.5 ± 0.5 and 32.5 ± 0.5 degrees, respectively (P>0.05). Positive amplitudes of SEAs were 3.71 ± 1.06 and 2.27 ± 0.69 mV at 36.5 ± 0.5 and 32.5 ± 0.5 degrees, respectively (P<0.001). Half-amplitude widths of SEAs were 47.26 ± 11.58 and 46.28 ± 13.41 ms at 36.5 ± 0.5 and 32.5 ± 0.5 degrees, respectively (P>0.05). These results indicate that temperature significantly affects only post-traumatic SEAs amplitudes. Furthermore, the previously reported SEAs likely reflect a post-traumatic mechanism rather than hyperexcitability caused by relatively high recording temperatures.
obtained from rats at 1-day post F-P brain injury. Extracellular recordings were performed in CA3c of slices from the hippocampus ipsilateral to the F-P brain injury. SEAs were recorded in 64 ± 15% (mean ± SD) of tested slices from 5 rats at 36.5 ± 0.5 degrees, and in 60 ± 14% of tested slices from 4 rats at 32.5 ± 0.5 degrees (P>0.05). Frequencies of SEAs were 0.08 ± 0.03 (n=16) and 0.07 ± 0.04 Hz (n=12) at 36.5 ± 0.5 and 32.5 ± 0.5 degrees, respectively (P>0.05). Positive amplitudes of SEAs were 3.71 ± 1.06 and 2.27 ± 0.69 mV at 36.5 ± 0.5 and 32.5 ± 0.5 degrees, respectively (P<0.001). Half-amplitude widths of SEAs were 47.26 ± 11.58 and 46.28 ± 13.41 ms at 36.5 ± 0.5 and 32.5 ± 0.5 degrees, respectively (P>0.05). These results indicate that temperature significantly affects only post-traumatic SEAs amplitudes. Furthermore, the previously reported SEAs likely reflect a post-traumatic mechanism rather than hyperexcitability caused by relatively high recording temperatures.
Supported by Ontario Neurotrauma Foundation
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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