Neuroscience 2005 Abstract
| Presentation Number: | 426.16 |
|---|---|
| Abstract Title: | Analysis of proteasome function in the CNS of young and old <i>Drosophila.</i> |
| Authors: |
Jansen, M. T.*1
; Rogoz, A.1
; Vernace, V.1
; Yeh, C.1
; Kim, J.1
; Ma, D.1
; Figueiredo-Pereira, M.1
; Schmidt-Glenewinkel, T.1
1Biological Sciences, Hunter Col. and GC of CUNY, New York, NY |
| Primary Theme and Topics |
Disorders of the Nervous System - Neurodegenerative and Movement Disorders -- Parkinson's disease: Models |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Neurodegenerative and Movement Disorders<br />-- Parkinson's disease: Cells, circuits, and systems |
| Session: |
426. Parkinson's Disease: Mitochondria and Proteosome Function Poster |
| Presentation Time: | Monday, November 14, 2005 11:00 AM-12:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # NN23 |
| Keywords: | Parkinson's, Ubiquitination, Degron, P-element |
Neurodegenerative disorders are characterized by intracellular and extracellular protein aggregates. Analysis of the Lewy bodies found in Parkinson’s disease (PD) and the neurofibrillary tangles found in Alzheimer’s disease (AD) demonstrate that these protein aggregates contain ubiquitinated proteins. The apparent inability of these neurons to degrade ubiquitinated proteins may arise from a malfunction of the ubiquitin proteasome pathway (UPP). To further investigate the possible relationship between neuronal protein aggregates and the ubiquitin proteasome pathway we have constructed a transgenic Drosophila model which allows us to monitor the activities of the UPP in vivo. Using modified P-element vectors, pGreen H-Pelican and pGreen Pelican, (a gift of Dr. Posakony, UCSD) both of which contain GFP, we were able to create a GFP-CL1 degron (ACKNWFSSLSHFVIHL) reporter with the degron attached to the carboxyl terminal of GFP. Using the Drosophila promoters embryonic lethal adult visual system (ELAV) and dopamine decarboxylase (DDC) we were able to express the fusion protein in the general neuronal population and the dopaminergic neurons of the nervous system. The GFP-CL1 fusion protein reduces the half life of GFP thereby allowing us to effectively monitor UPP activity in both young and old Drosophila.
Supported by NIGMS-GM60654 to T.S.-G. and M.E.F.-P, PSC-CUNY 66242-00 35 to T.S.-G. NIH (NINDS-NS34018 to M.E.F.-P and NCRR-RR03037 to Hunter College of CUNY) and the City University of New York.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
Copyright © 2005-2026 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
