Neuroscience 2004 Abstract
| Presentation Number: | 388.18 |
|---|---|
| Abstract Title: | Association of fragile X mental retardation protein with stress granules or polyribosomes after synaptic stimulation. |
| Authors: |
Kim, S.*1
; Yeshwant, S.1
; Dong, W. K.1
; Weiler, I. J.1
; Greenough, W. T.1
1Beckman Inst., Univ. of Illinois, Urbana-Champaign, Urbana, IL |
| Primary Theme and Topics |
Development - Synaptogenesis and Activity-Dependent Development -- Activity-dependent development and plasticity |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Intracellular Signaling Pathways<br />-- Second messengers |
| Session: |
388. Activity-Dependent Development and Plasticity IV Poster |
| Presentation Time: | Monday, October 25, 2004 9:00 AM-10:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # E36 |
| Keywords: | translation, granules, synapse, FMRP |
Fragile X Mental Retardation protein (FMRP), which is not produced in victims of Fragile X Syndrome, is a mRNA binding protein that seems to be involved in transport and translational regulation of a subset of mRNAs. We have continued our investigations into the FMRP-containing stress granules (Mazroui et al., 2002) which form in response to in vivo electrical stimulation of hippocampal neurons (Weiler et al, SfN 2003). Since the granule fraction defined by centrifugation yields a motley collection of mRNP and transport granules, we have chosen to use TIA-1 as a marker to select stress granules. Hippocampi from rats before and at defined intervals after in vivo perforant path stimulation are rapidly removed and frozen on dry ice. After cell lysis, granules are rapidly and gently purified using antibody to TIA-1 that is conjugated to magnetic beads. The protein content of stress granules can then be directly compared to the proteins associated with a polyribosomal pellet isolated from the same lysate through a 1M sucrose cushion.
Supported by IEI Kiwanis
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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