Neuroscience 2001 Abstract
| Presentation Number: | 434.2 |
|---|---|
| Abstract Title: | The permeability of the blood brain barrier differs in immature and mature rats following transient focal cerebral ischemia. |
| Authors: |
Wendland, M.*1
; Derugin, N.2
; Manabat, C.3
; Vexler, Z.3
1Radiology, University California, San Francisco, San Francisco, CA 2Neurosurgery, University California, San Francisco, San Francisco, CA 3Neurology, University California, San Francisco, San Francisco, CA |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Ischemia |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Cerebral Blood Flow |
| Session: |
434. Ischemia: cellular and molecular mechanisms X Poster |
| Presentation Time: | Monday, November 12, 2001 2:00 PM-3:00 PM |
| Location: | Exhibit Hall XX-50 |
| Keywords: | middle cerebral artery occlusion, MRI, stroke |
Neonatal stroke is an important cause of cognitive deficits, cerebral palsy, and epilepsy. The vulnerability and mechanisms of neonatal cerebral ischemia-reperfusion may differ from that in the mature cerebral nervous system. To determine if increased permeability of the blood-brain barrier (BBB) contributes significantly to the acute ischemic injury in neonatal rats, we subjected postnatal day 7 (P7) rats to a 3 hr transient middle cerebral artery (MCA) occlusion and evaluated the integrity of the BBB by using T1W MRI in conjunction with GdDTPA (0.3mmol/kg) at 4, 7 and 24 hr after reperfusion. To compare the patterns of post-ischemic BBB permeability in P7 versus adult rat brains, young adult rats (n=3) were subjected to 3hr MCA occlusion followed by reflow. Each animal was examined by DW-MRI (for cytotoxic edema), T2W-MRI (for vasogenic edema) and dynamic T2W* MRI in conjunction with contrast bolus (perfusion-sensitive), T1W MRI. Evans Blue was injected (i.v.) and animals sacrificed immediately following MRI, 7 or 24 hr after reperfusion. All animals exhibited DW-MRI hyperintensity during occlusion and reperfusion, in the area of occluded MCA. T2W hyperintensity was evident at 24 hr in all cases, but not at 4 hr post-reflow in P7 rats. None of the neonates showed Gd-DTPA uptake at 24 hr, and leakage of Evans Blue was not evident. In contrast, adult animals all showed Gd-DTPA uptake at 24 hr. These results show greater BBB leakage in adults vs. neonates and support differences in mechanisms of post-reperfusion injury for the mature and immature brain.
Supported by AHA, Western States Affiliates #9900044
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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