Neuroscience 2001 Abstract
| Presentation Number: | 430.21 |
|---|---|
| Abstract Title: | EFFECTS OF FORCED LIMB USE ON BEHAVIORAL OUTCOME AND EXPRESSION OF FGF-2 AFTER PARTIAL UNILATERAL 6-OHDA LESIONS OF NIGROSTRIATAL DOPAMINE NEURONS. |
| Authors: |
Stewart, J.*1
; Moroz, I.1
; Cohen, A. D.2
; Tillerson, J. L-.2
; Martinez, E.1
; Schallert, T.2,3
1CSBN, Concordia Univ, Montreal, Canada 2Inst Neurosci, Univ Texas, Austin, TX 3Univ Michigan, Ann Arbor, MI |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Neurodegenerative Disorders -- Parkinsons DIsease: Models |
| Session: |
430. Neurodegenerative disorders: Parkinson's disease--neurotoxins Poster |
| Presentation Time: | Monday, November 12, 2001 1:00 PM-2:00 PM |
| Location: | Exhibit Hall WW-67 |
| Keywords: | bFGF, TH, immunoreactivity, MFB |
Basic fibroblast growth factor (FGF-2) has neurotrophic and neuroprotective effects on midbrain dopamine (DA) cells. Astrocytic expression of FGF-2 is increased in the cell body region of these neurons, ipsilateral to 6-OHDA lesions (Moroz & Stewart, SFN Abst 2000). Recently, Tillerson et al (2001) showed that forced use of the impaired limb after such lesions results in long-term protection of striatal DA tissue content and limb use. Here we examined the impact of forced use of the impaired forelimb after unilateral 6-OHDA nigrostriatal lesions on FGF-2 expression. At the time of lesioning rats were fitted with casts forcing them to rely on either the impaired (IPSI-cast) or non-impaired (CONTRA-cast) limb for 7 days, or were left uncasted (NO-cast); there were also two Sham lesion groups (Cast and NO-cast). Two days after cast removal, rats were tested for asymmetry of limb use. Sham and IPSI-casted rats showed no impairment or asymmetry, whereas Contra-cast and NO-cast lesioned rats did. Lesions reduced TH-IR cells in SNc and VTA by about 50 % in all groups. The number of TH-IR cells in SNc was negatively correlated with FGF-2-IR and behavioral impairment. Both lesion and use alone increased FGF-2-IR cells in SNc and VTA (ipsilateral to the lesion and on both sides in Sham-Cast animals), but the effects were not additive. Finally, although TH-IR cells were not significantly spared by forced use, this does not preclude enhanced functioning of DA neurons at later time points as reported by Tillerson et al, 2001.
Supported by NINDS and CIHR (Canada)
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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