Neuroscience 2001 Abstract
| Presentation Number: | 426.2 |
|---|---|
| Abstract Title: | A STANDARDIZED METHOD FOR BRAIN-CUTTING SUITABLE FOR BOTH MRI-BRAIN CO-REGISTRATION AND STEREOLOGICAL MEASURES. |
| Authors: |
Zarow, C.*1,2
; Kim, T. S.1
; Chui, H. C.1,2
1University of Southern California, Los Angeles, CA 2Rancho Los Amigos National Rehabilitation Center, Downey, CA |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Neurodegenerative Disorders -- Alzheimers Disease: Other |
| Session: |
426. Neurodegenerative disorders: Alzheimer's disease--imaging and pathology II Poster |
| Presentation Time: | Monday, November 12, 2001 2:00 PM-3:00 PM |
| Location: | Exhibit Hall VV-52 |
| Keywords: | STEREOLOGY, postmortem MRI |
Both co-registration of the in vivo MRI to postmortem brain for MRI-pathological correlations, as well as sampling of brain tissue for stereological measures, require precisely spaced slices of the human brain. We have developed an agar-embedding method for brain-slicing that minimizes geometrical distortions from slicing and handling the fixed postmortem brain. To facilitate MRI co-registration, each hemisphere is handled separately. We embed the fixed brain hemisphere, with plastic reference markers, in agar. The block containing the brain and markers is sliced at 5 mm intervals using a rotary slicer. Each slice is photographed with a high-resolution digital camera. The digital images are reconstructed as a 3-dimensional volume via a point-based registration method for multi-slice registration. The slices of the reconstructed postmortem hemisphere are then co-registered to corresponding slices of an in vivo reference MRI-volume (3-D T1-weighted coronal SPGR, 1.4 mm slice thickness) using polynomial warping by maximizing mutual information. We are using these MRI-brain co-registration methods to correlate in vivo T2-weighted MRI hyperintensities in gray and white matter with underlying pathology. For design-based stereology, the volume of interest (VOI) is defined using reproducible anatomical boundaries. Tissue is sampled systematically by examining every nth section from each 5 mm brain slice containing the VOI. We are using these methods to estimate numbers of neurons in the hippocampus and oligodendrocytes in normal and abnormal white matter in Alzheimer disease and vascular dementia.
Supported by NIH P01-AG12435
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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