Neuroscience 2002 Abstract
Presentation Number: | 404.13 |
---|---|
Abstract Title: | Functional magnetic resonance imaging of amphetamine. |
Authors: |
Dixon, A. L.*1
; Shah, Y. B.2
; Prior, M.2
; Morris, P. G.2
; Joseph, M. J.1
; Young, A. M. J.1
1School of Psychology, University of Leicester, Leicester, United Kingdom 2School of Physics, University of Nottingham, Nottingham, United Kingdom |
Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
Secondary Theme and Topics | Autonomic, Limbic and Other Systems<br />- Brain Metabolism, Transport and Blood Flow |
Session: |
404. Staining, tracing, and imaging techniques IV Poster |
Presentation Time: | Monday, November 4, 2002 1:00 PM-2:00 PM |
Location: | Hall A2-B3 Z-38 |
Keywords: | DOPAMINE RECEPTOR, ANTAGONIST, MRI, RAT |
We used pharmacological MRI (phMRI) to study dopamine (DA) systems in the rat brain, by measuring changes in local brain activity after administration of amphetamine (Amp) with and without two other dopaminergic drugs. For phMRI Amp is idea as a tool, due to its well-characterised and robust response as well being an indirect DA agonist (on both D1 and D2-type of DA receptors). Thus by manipulating Amp stimulation using specific D1 and D2 antagonists, one may be able to ascertain for which components of the phMRI response the receptor types are responsible, with the ultimate goal of gaining information on the nature of the BOLD signal regarding transmitter interaction.
T2-weighted fMRI (2.35T Bruker system) was used to identify the pattern and determinants of Amp-induced brain activation in drug-naïve rats (3mg/kg, i.v, n=8). Additionally we investigated the effect of pre-treating individual cohorts of rats with D1 antagonist SCH23390(0.5mg/kg, i.v, n=8) or sulpiride (50mg/kg, i.v, n=8) before acute challenge of Amp. Statistical analysis was conducted and significance determined by a Student paired t-test using SPM’99 software (Institute of Neurology, London, U.K). SPM analysis showed Amp to cause significant signal increases in many brain regions including PAG, striatum, globus pallidus, cingulate cortex and hippocampus (CA3) and decreases in many brain regions including the primary motor cortex, hypothalamus and substantia nigra. Neither pre-treatments blocked the response to Amp completely, instead each drug had an effect on the increase or decrease component of the Amp response and not both, as previously observed.
T2-weighted fMRI (2.35T Bruker system) was used to identify the pattern and determinants of Amp-induced brain activation in drug-naïve rats (3mg/kg, i.v, n=8). Additionally we investigated the effect of pre-treating individual cohorts of rats with D1 antagonist SCH23390(0.5mg/kg, i.v, n=8) or sulpiride (50mg/kg, i.v, n=8) before acute challenge of Amp. Statistical analysis was conducted and significance determined by a Student paired t-test using SPM’99 software (Institute of Neurology, London, U.K). SPM analysis showed Amp to cause significant signal increases in many brain regions including PAG, striatum, globus pallidus, cingulate cortex and hippocampus (CA3) and decreases in many brain regions including the primary motor cortex, hypothalamus and substantia nigra. Neither pre-treatments blocked the response to Amp completely, instead each drug had an effect on the increase or decrease component of the Amp response and not both, as previously observed.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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