Neuroscience 2002 Abstract
| Presentation Number: | 404.10 |
|---|---|
| Abstract Title: | Diffusion MRI of Rat Hippocampal Slice Cultures: Initial Results. |
| Authors: |
Shepherd, T. M.*1
; King, M. A.1
; Grant, S. C.1
; Thelwall, P. E.1
; Blackband, S. J.1
; Wirth, E. D.2
1Neuroscience, University of Florida, Gainesville, FL 2Neurosurgery, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Session: |
404. Staining, tracing, and imaging techniques IV Poster |
| Presentation Time: | Monday, November 4, 2002 2:00 PM-3:00 PM |
| Location: | Hall A2-B3 Z-38 |
| Keywords: | BRAIN SLICE, TISSUE CULTURE, IMAGING, HIPPOCAMPUS |
Brain slices provide a controllable in situ model for investigating the biophysical basis of water diffusion in nervous tissue. Yet only acute studies are possible because brain slices seldom remain viable beyond 12 hrs due to cell death processes initiated by procurement. This limitation may be overcome by developing diffusion MRI methods for organotypic cultures, which remain viable for many weeks. In the present study, hippocampal slices were procured from P7 rat pups and cultured for 10 days on Millipore culture plate inserts. The inserts then were placed into an NMR tube and imaged using a 15mm birdcage coil in a 750-MHz wide-bore spectrometer. Although the small volume of cultured slices (~ 0.8 mm3) limited available signal-to-noise (S/N), in 1 slice, 8 diffusion-weighted images with b values between 56 and 3970 s/mm2 were obtained with S/N ratios of at least 18. As a first approximation, biexponential analysis of diffusion-weighted signal attenuation in this slice resulted in values of 0.54 for Ffast, 1.40 x 10-3 mm2/s for D fast and 0.11 x 10-3 mm2/s for Dslow. These values are comparable to those previously reported for acute brain slice preparations. Future improvements in sample preparation and coil utilization will help to obtain adequate image S/N from slice cultures on a consistent basis. This technique may soon facilitate investigations into the biological origins of diffusion which require longer stability than possible in acute brain slice preparations.
Supported by Florida Brain and Spinal Cord Injury Research Trust Fund
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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