Neuroscience 2002 Abstract
| Presentation Number: | 404.7 |
|---|---|
| Abstract Title: | Assessment of Ibotenic Acid Amygdala Lesions in Monkeys Using MRI. |
| Authors: |
Shelton, S. E.*1
; Oakes, T. R.3
; Kalin, N. H.1,2
1Psychiatry, University of Wisconsin, Madison, WI 2Psychology, University of Wisconsin, Madison, WI 3W. M. Keck Laboratory for Functional Brain Imaging, University of Wisconsin, Madison, WI |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Session: |
404. Staining, tracing, and imaging techniques IV Poster |
| Presentation Time: | Monday, November 4, 2002 3:00 PM-4:00 PM |
| Location: | Hall A2-B3 Z-38 |
| Keywords: | IMAGING, PRIMATE, NEUROTOXICITY |
We have embarked on a series of studies lesioning the amygdala in rhesus monkeys to understand its involvement in mediating fear-related psychopathology. Ibotenic acid lesions are preferable because they allow for selective damage and spare fibers of passage. Since these experiments are long term, relying on traditional histopathological verification of lesion extent is problematic. Therefore, we have been exploring noninvasive methods to determine the accuracy and extent of lesions. To assess the validity of using MRI to verify lesions, T2-weighted images were obtained from 5 monkeys at 1,2, and 3 weeks after lesioning. Approximately 9-12 months after surgery, 40 micron thick histological brain sections were obtained, and the extent of histopathological damage was compared with the one week post-surgery MRI data. A Region-of-Interest (ROI) was drawn around the bright signal region on the T2-weighted MRI (indicative of edema). Then the region of actual tissue loss based on each individual's histological data was drawn. The histology images were then coregistered to the T2/MRI image using a manual coregistration package. The lesion as defined by histology was on average 47% of the volume indicated by the T2/MRI signal. The edematous areas indicated by T2/MRI data acquired at 2 and 3 weeks post-surgery were more variable than those acquired 1 week post-surgery. These data demonstrate the utility of post-surgical T2-weighted MRIs in estimating the success of neurotoxic lesions in primates. While the MRI assessments tend to overestimate lesion size, they provide an early indication of accuracy.
Supported by MH46729 and the HealthEmotions Research Institute
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
Copyright © 2002-2025 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
