Neuroscience 2002 Abstract
| Presentation Number: | 404.6 |
|---|---|
| Abstract Title: | Using MRI to monitor tumor formation and progression in a mouse model of neurofibromatosis type I plexiform neurofibroma. |
| Authors: |
Perrin, G.*1
; Walton, R.2
; Mareci, T.2
; Muir, D.1,3
1Dept. of Neuroscience, Univ. of Florida, Gainesville, FL 2Dept. of Biochemistry and Molecular Biology, Univ. of Florida, Gainesville, FL 3Dept. of Pediatric Neurology, Univ. of Florida, Gainesville, FL |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Session: |
404. Staining, tracing, and imaging techniques IV Poster |
| Presentation Time: | Monday, November 4, 2002 2:00 PM-3:00 PM |
| Location: | Hall A2-B3 Z-38 |
| Keywords: | nerve degeneration, peripheral nerve tumor, IMAGING |
Plexiform neurofibromas in patients with neurofibromatosis type I (NF1) typically involve deep or named nerves, can become very large, may cause serious functional impairment and have a risk of malignant progression. In developing an animal model to study plexiform neurofibromas, we have shown that human NF1 neurofibrosarcoma-derived Schwann cells (SC) can be successfully xenografted into the sciatic nerves of scid mice. The histogenesis of engrafted neurofibrosarcoma SC was consistent with that of established human plexiform neurofibromas. The tumors invaded and degenerated the host nerve structure and proliferated in a slow, sustained manner without apparent malignancy. In addition, we observed angiogenic markers in xenografted nerves. We performed MRI to study the formation and progression of these tumors in our model. Using a 17.6 tesla, 89mm bore magnet we performed H-1 MRI at 750MHz on excised, fixed xenografted mouse sciatic nerves. We then compared the T1 weighted images to immunostaining of the same nerves with an antibody specific to human glutathione S-transferase. The hypointense areas of nerve seen in the MRI data corresponded to the areas staining positive for human tumor cells. Similar to human plexiform neurofibromas, demyelinization and increased extracellular matrix were also observed and likely contributed to the effects seen in the MRI data. Therefore, we can use MRI to monitor tumor development and progression. We are currently using MRI to further investigate tumor progression and angiogenesis in vivo.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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