Neuroscience 2002 Abstract
| Presentation Number: | 404.3 |
|---|---|
| Abstract Title: | INTRAVENTRICULAR HEMORRHAGE: MRI AND HISTOLOGICAL STUDY IN THE NEONATAL MOUSE CEREBRUM. |
| Authors: |
Xue, M.*1,2
; Buist, R.3
; Del Bigio, M. R.1,2
1Pathology, Univesity of Manitoba, Winnipeg, Canada 2Human Anatomy, Univesity of Manitoba, Winnipeg, Canada 3Pharmacology, Univesity of Manitoba, Winnipeg, Canada |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Session: |
404. Staining, tracing, and imaging techniques IV Poster |
| Presentation Time: | Monday, November 4, 2002 3:00 PM-4:00 PM |
| Location: | Hall A2-B3 Z-38 |
| Keywords: | intracerebral hemorrhage , magnetic resonance images , cell death, inflammation |
Introduction: In the brain of premature infants primary intracerebral hemorrhage (ICH) is a common occurrence. It is usually unilateral and deep in the cerebrum near the ganglionic eminence beside the lateral ventricle. Blood can extend into the ventricles causing hydrocephalus. Even small lesions of this type, are associated with poor developmental outcome including cerebral palsy and mental retardation. The purpose of this study was to obtain magnetic resonance images (MRI) of the mouse brain after ICH and to correlate the images with histological evolution.
Methods: ICH was induced in one-day-old mice, which are developmentally similar to a 24-26 weeks human fetus, by injection of autologous blood into the striatum. MRI was obtained 15 minutes to 48 hours later. Mice were perfused with 4% paraformaldehyde 8 hours to 28 days later. H&E, immunohistochemical, and TUNEL staining were used to quantify the lesion area, neutrophils, microglia, and cell death at the edge of the hemorrhagic lesion.
Results: Histological correlation showed that MRI is capable of resolving the hematoma, but the MRI showed larger hematoma area than the H&E stain. This is likely due to susceptibility artifact. H&E stain showed that blood was located in the striatum or germinal tissue and often entered the ventricle. Neutrophils peaked at 2-3 days, microglia reaction appeared at 2 days persisted up to 28 days.
Conclusion: MRI correlates reasonably well with histological changes in this ICH model. The inflammatory response may contribute to brain injury at the periphery of the hematoma.
Methods: ICH was induced in one-day-old mice, which are developmentally similar to a 24-26 weeks human fetus, by injection of autologous blood into the striatum. MRI was obtained 15 minutes to 48 hours later. Mice were perfused with 4% paraformaldehyde 8 hours to 28 days later. H&E, immunohistochemical, and TUNEL staining were used to quantify the lesion area, neutrophils, microglia, and cell death at the edge of the hemorrhagic lesion.
Results: Histological correlation showed that MRI is capable of resolving the hematoma, but the MRI showed larger hematoma area than the H&E stain. This is likely due to susceptibility artifact. H&E stain showed that blood was located in the striatum or germinal tissue and often entered the ventricle. Neutrophils peaked at 2-3 days, microglia reaction appeared at 2 days persisted up to 28 days.
Conclusion: MRI correlates reasonably well with histological changes in this ICH model. The inflammatory response may contribute to brain injury at the periphery of the hematoma.
Supported by CIHR, HSFC, and MHRC
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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