Neuroscience 2002 Abstract
| Presentation Number: | 398.11 |
|---|---|
| Abstract Title: | Affymetrix expression profiling in cortical regions of rats with high and low anxiety-like behavior. |
| Authors: |
Arlinde, C.*1
; Sommer, W.1
; Kalish, R.2
; Wigger, S.3
; Auer, D.2
; Landgraf, R.3
; Heilig, M.1
1NEUROTEC, Karolinska Institute, Huddinge, Sweden 2NMR, Max Plank Institute of Psychiatry, Munich, Germany 3Behavioral Neuroendocrinology, Max Plank Institute of Psychiatry, Munich, Germany |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Psychiatric Disorders -- Anxiety disorders |
| Session: |
398. Psychiatric disorders: anxiety disorders III Poster |
| Presentation Time: | Monday, November 4, 2002 3:00 PM-4:00 PM |
| Location: | Hall A2-B3 Y-10 |
| Keywords: | GENE EXPRESSION, MRI, CORTEX, PLUS MAZE |
Extreme anxiety-like behavior and pronounced responsiveness to the anxiolytic effects of benzodiazepines are characteristic features of genetically selected HAB (high anxiety-like behavior) rats which can be regarded as modeling aspects of human anxiety disorders. Here, we analyze expression profiles from prefrontal and parietal cortex of naïve HAB rats and controls. The regions were chosen based on our recent findings demonstrating a strong differential pharmacological MRI (phMRI) response to diazepam between HAB rats and their genetic counterpart strain (LAB) in the medial prefrontal/anterior cingulatecortex (Kalisch et al, SFN 2001).
Inbred HAB and LAB rats (n=9/group), characterized for their anxiety-like behavior on the elevated plus maze, were sacrificed and total RNA from cingulate and parietal cortex was prepared, pooled in groups of 3 individuals and hybridized to Affymetrix Rat Neurobiology Chip (U34) which contains probe sets for about 1200 genes. Strain and regional effects were analyzed. Selected candidate genes were confirmed by TaqMan real-time RT-PCR, using cDNA preparations from the individual RNA samples.
Preliminary data show that within the cingulate cortex 9 genes were differentially expressed between the HAB and LAB strains. Among these candidates, several appear to be involved in mediating the pharmacological action of benzodiazepines.
Inbred HAB and LAB rats (n=9/group), characterized for their anxiety-like behavior on the elevated plus maze, were sacrificed and total RNA from cingulate and parietal cortex was prepared, pooled in groups of 3 individuals and hybridized to Affymetrix Rat Neurobiology Chip (U34) which contains probe sets for about 1200 genes. Strain and regional effects were analyzed. Selected candidate genes were confirmed by TaqMan real-time RT-PCR, using cDNA preparations from the individual RNA samples.
Preliminary data show that within the cingulate cortex 9 genes were differentially expressed between the HAB and LAB strains. Among these candidates, several appear to be involved in mediating the pharmacological action of benzodiazepines.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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