Neuroscience 2000 Abstract
| Presentation Number: | 384.6 |
|---|---|
| Abstract Title: | Influence of different anesthesia conditions on functional magnetic resonance imaging during bicuculline-induced seizures in rats. |
| Authors: |
Abo, M.*1,4
; Chen, Z.1
; Prunell, G.2
; Lai, L. J.1
; Klason, T.1
; Miyano, S.4
; Yonemoto, K.5
; Reese, T.3
; Bjelke, B.1
1MR Center, Karolinska Institutet, Stockholm, Sweden 2Neurosurgery, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden 3Biol. NMR, SmithKline Beecham, Herts, United Kingdom 4Dept. Rehab Medicine, Jikei University School of Medicine, Jikei, Japan 5Tokyo Metropolitan University of Health Science, Tokyo, Japan |
| Primary Theme and Topics |
J. Disorders of the Nervous System and Aging - 135. Ischemia: cerebral blood flow |
| Secondary Theme and Topics | H. Other Systems of the CNS<br />- 101. Brain metabolism and blood flow |
| Session: |
384. Ischemia: cerebral bloodflow II Poster |
| Presentation Time: | Monday, November 6, 2000 2:00 PM-3:00 PM |
| Location: | Hall G-J |
| Keywords: | ISCHEMIA, HINDLIMB, MOTOR CORTEX, BEHAVIOR |
It is highly desirable to measure neuronal activity by mapping the changes in local cerebral blood volume (CBV) even though this is an indirect method. We wanted to further explore the effects of physiological properties that might influence the results obtained in functional magnetic resonance (fMRI) studies. Male Sprague Dawley rats b.wt. 200 to 230 g were used. The MRI recordings were performed on a Biospec Avance 47T spectrometer with a 12 cm gradient system, 200 mT/m in combination with a 35 mm RF resonator. The conditions studied were; (n=3 rats per group), (1) Isoflurane 1.5% in Air, (2) Isoflurane 2.0% in Air,(3) Isoflurane 1.5% in 60% oxygen (O2 and 40% nitrous oxide (N2O), (4) Isoflurane 2.0% in 60% O2 and 40% N2O, (5) Isoflurane 1.5% in 40% O2 and 60% nitrogen (N2, (6) Isoflurane 2.0% in 40% O2 and 60% N2. Arterial PO2, PCO2, pH and Blood pressure, before and after bicuculline stimulation were measured. Under the studied conditions the best reaction of bicuculline stimulation, indeed, was 1.5% isoflurane in 60% nitrogen and 40% oxygen. Thus, this study shows that the transient changes in relative CBV induced by pharmacological stimulation of bicuculline is clearly effected by anaesthesia and must be considered when designing fMRI studies. This result implies that the rCBV variations using this level of isoflurane anaesthesia and the body temperature kept at 37.5°C could be suitable for further fMRI studies. Acknowledgements: Trygg Hansas Forskningsstiftelse, David och Astrid Hageléns stiftelse, Stroke-Förbundets Stiftelser och Fonder.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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