Neuroscience 2005 Abstract
| Presentation Number: | 358.1 |
|---|---|
| Abstract Title: | Perfusion MRI analysis of cerebral blood volume and ischemic damage following unilateral cerebral hypoxia--ischemia in aged mice. |
| Authors: |
Vannucci, S. J.*1
; Pichiule, P.1
; Aleshin, A.3
; Ferrazzano, P.1
; Ramasamy, R.3
; Schmidt, A.3
; Small, S. A.2
1Pediatrics, Columbia Univ. Col. of Physicians and Surgeons, New York, NY 2Neurology, Columbia Univ. Col. of Physicians and Surgeons, New York, NY 3Surgery, Columbia Univ. Col. of Physicians and Surgeons, New York, NY |
| Primary Theme and Topics |
Disorders of the Nervous System - Ischemia -- Human studies and animal models |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Aging<br />-- Physiology |
| Session: |
358. Ischemia: Human Studies Slide |
| Presentation Time: | Monday, November 14, 2005 8:00 AM-8:15 AM |
| Location: | Washington Convention Center - Room 150B |
| Keywords: |
Aging is associated with an increased risk for stroke and enhanced injury with reperfusion, although the aging-specific mechanisms underlying these observations are not known. Stroke studies in aged (>20 month old) transgenic mice are needed to address these questions, including potential alterations in cerebral blood flow (CBF). The purpose of this study was to utilize perfusion MRI to determine the effects of hypoxia-ischemia (H/I) on cerebral blood volume (CBV) as a measure of CBF, as well as post-ischemic structural changes, in young (3 month) and aged (24 month) mice. Male C57Bl/6 mice were subjected to unilateral carotid artery ligation (isoflurane anesthesia), recovered 2 hours, injected ip with gadolinium pentate (10mmol/Kg), and positioned in a small bore 9.4 tesla vertical Brucker magnet for multiple whole brain T2 images. Following baseline, 8% O2/bal N2 was delivered to obtain H/I images as depicted below. Top panel are CBV maps with corresponding structural images below, ligated(ischemic) hemisphere on left: baseline, 10 min, 20 min H/I, demonstrating significant reductions in end-ischemic CBV. Bottom panel depicts corresponding sturctural images. Animals imaged 1-5 days later show ischemic damage as confirmed with H&E stains. The results of this study show no baseline CBV differences between young and old mice but structural changes associated with aging. Aged mice survive this insult and subsequent MRI. This model of H/I is well suited for stroke studies in aged, 24 month old mice.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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