Neuroscience 2002 Abstract
| Presentation Number: | 388.14 |
|---|---|
| Abstract Title: | Stem cell transplantation in the 3-NPA rat model of Huntington's disease: Time course by MRI. |
| Authors: |
Roberts, T. J.*1
; Sandhu, J.2
; Modo, M. M.1
; Molloy, G.2
; Price, J.2
; Williams, S. C. R.1
1Neuroimaging Research Group, Institute of Psychiatry, De Crespigny Park, London, United Kingdom 2Department of Neuroscience, Institute of Psychiatry, De Crespigny Park, London, United Kingdom |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Neurodegenerative Disorders -- Trinucleotide Repeat Diseases: Huntingtons Disease |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, tracing and imaging techniques |
| Session: |
388. Neurodegenerative disorders: trinucleotide repeat diseases--Huntington's disease IV Poster |
| Presentation Time: | Monday, November 4, 2002 2:00 PM-3:00 PM |
| Location: | Hall A2-B3 T-12 |
| Keywords: | NITROPROPIONIC, NEUROIMAGING, NEURODEGENERATION, GRAFT |
In vivo assessment of stem cell transplantation by MRI will allow the evaluation of indirect markers of graft effects over time. Current clinical evaluation of fetal tissue transplants is achieved using a battery of behavioural and neuroimaging techniques. Application of neuroimaging techniques to stem cell grafts will be more problematical due to the diffuse nature of graft integration caused by cell migration into the host parenchyma. Therefore, more sensitive neuroimaging methods need to be developed to assess the effects of such cells in the clinical domain.
Using serial T1, T2, T2* and proton density weighted MRI scans at 2 days pre-grafting and 2, 7, 14 and 56 days post-grafting we have measured the effects of unilateral grafting of conditionally immortalised MHP-36 stem cells (2 deposits of 50000 cells in 4μL NAC) on the systemic 3-NPA rat model of Huntington’s disease. Changes relative to pre-grafting in total brain volume, CSF volume and total tissue volume were measured. Temporal evolution of these measures in vehicle and MHP-36 transplanted rats follow a similar pattern with no significant differences between groups at any time point. However at later time points, MHP-36 treated animals showed a trend to greater total brain volume (p=0.14, n=3 per group) in comparison to vehicle treated rats suggesting that detection of indirect effects of stem cell transplants might take longer than 2 months after grafting.
Using serial T1, T2, T2* and proton density weighted MRI scans at 2 days pre-grafting and 2, 7, 14 and 56 days post-grafting we have measured the effects of unilateral grafting of conditionally immortalised MHP-36 stem cells (2 deposits of 50000 cells in 4μL NAC) on the systemic 3-NPA rat model of Huntington’s disease. Changes relative to pre-grafting in total brain volume, CSF volume and total tissue volume were measured. Temporal evolution of these measures in vehicle and MHP-36 transplanted rats follow a similar pattern with no significant differences between groups at any time point. However at later time points, MHP-36 treated animals showed a trend to greater total brain volume (p=0.14, n=3 per group) in comparison to vehicle treated rats suggesting that detection of indirect effects of stem cell transplants might take longer than 2 months after grafting.
Supported by BBSRC, MRC
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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