Neuroscience 2005 Abstract
| Presentation Number: | 337.10 |
|---|---|
| Abstract Title: | Behavioral and neuropathological changes after administration of nimodipine, magnesium chloride, and magnesium sulphate in kaolin-induced neonatal rat hydrocephalus. |
| Authors: |
Khan, O. H.*1,2
; Del Bigio, M.1,2
1Pathology, Univ. of Manitoba, Winnipeg, Canada 2MB, D212-770 Bannatyne Ave., R3E0W3, |
| Primary Theme and Topics |
Disorders of the Nervous System - Neurotoxicity, Inflammation, and Neuroprotection -- Neuroprotective mechanisms and treatments |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Demyelinating Disorders<br />-- Human studies and animal models |
| Session: |
337. Neuroprotective Mechanisms and Treatments II Poster |
| Presentation Time: | Sunday, November 13, 2005 2:00 PM-3:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # VV41 |
| Keywords: | myelin, protection, axon, calcium |
O. Khan, M.R. Del Bigio* Department of Pathology, University of Manitoba
Hydrocephalus is a common neurological condition characterized by obstruction of cerebrospinal fluid (CSF) flow leading to enlargement of CSF-containing ventricular cavities in the brain. The use of pharmacological drugs to reduce brain damage in immature (5 – 7 weeks) hydrocephalic rats have been investigated in our lab with various outcomes (Del Bigio 2002). Magnesium sulphate and nimodipine have shown promising results with improvements in behavioral outcomes and reduced damage in the white matter (Del Bigio 2001, Khan 2003). However, shunting delay is more likely to be a clinical problem in very premature infants. Hence our desire to study the effects of these agents in neonatal rats with brains more comparable to those of 24-26 week humans.
Rats were injected with kaolin on postnatal day 1 and magnetic resonance imaging (MRI) was performed on day 7 to assess ventricle size. On postnatal day 7 osmotic mini pumps with MgSO4, MgCl2 (150 mg/kg/day), or nimodipine (15 or 30 mg/kg/day) were loaded and placed subcutaneously. Behavior tests for developmental delay were conducted at various time-points. Rotorod and Morris Water Maze were performed on day 20. MRI was conducted on day 21 for final ventricle size assessment. Pups were euthanized on day 21 and brains were subjected to histopathological and biochemical analyses. Nimodipine was of no benefit. Pups receiving MgCl2 were also of no benefit; however, the hyperosmolarity of the drug caused scab formation and possibly complicated results. MgSO4 experiments are currently being assessed.
Hydrocephalus is a common neurological condition characterized by obstruction of cerebrospinal fluid (CSF) flow leading to enlargement of CSF-containing ventricular cavities in the brain. The use of pharmacological drugs to reduce brain damage in immature (5 – 7 weeks) hydrocephalic rats have been investigated in our lab with various outcomes (Del Bigio 2002). Magnesium sulphate and nimodipine have shown promising results with improvements in behavioral outcomes and reduced damage in the white matter (Del Bigio 2001, Khan 2003). However, shunting delay is more likely to be a clinical problem in very premature infants. Hence our desire to study the effects of these agents in neonatal rats with brains more comparable to those of 24-26 week humans.
Rats were injected with kaolin on postnatal day 1 and magnetic resonance imaging (MRI) was performed on day 7 to assess ventricle size. On postnatal day 7 osmotic mini pumps with MgSO4, MgCl2 (150 mg/kg/day), or nimodipine (15 or 30 mg/kg/day) were loaded and placed subcutaneously. Behavior tests for developmental delay were conducted at various time-points. Rotorod and Morris Water Maze were performed on day 20. MRI was conducted on day 21 for final ventricle size assessment. Pups were euthanized on day 21 and brains were subjected to histopathological and biochemical analyses. Nimodipine was of no benefit. Pups receiving MgCl2 were also of no benefit; however, the hyperosmolarity of the drug caused scab formation and possibly complicated results. MgSO4 experiments are currently being assessed.
Supported by University of Manitoba and Manitoba Institute of Child Health
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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