Neuroscience 2005 Abstract
| Presentation Number: | 329.18 |
|---|---|
| Abstract Title: | Magnetic resonance labeling for human retinal pigment epithelial cell implantation: <i>in vitro</i> development of a potential method for <i>in vivo</i> follow-up. |
| Authors: |
Flores, J.*1
; Kozlowski, P.3
; O'Kusky, J. R.2
; MacKay, A.3
; Li, D.3
; Doudet, D. J.1
1Pacific Parkinson's Research Centre, Univ. of British Columbia, Vancouver, Canada 2Pathology, Univ. of British Columbia, Vancouver, Canada 3Radiology, Univ. of British Columbia, Vancouver, Canada |
| Primary Theme and Topics |
Disorders of the Nervous System - Neurodegenerative and Movement Disorders -- Parkinson's disease: Therapies |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, Tracing, and Imaging Techniques |
| Session: |
329. Parkinson's Disease: Cell-Based Therapies Poster |
| Presentation Time: | Sunday, November 13, 2005 2:00 PM-3:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # TT46 |
| Keywords: | Retinal Pigment Epithelial Cells, Parkinson's Disease, Imaging |
Intrastriatal implantation of human retinal pigment epithelial (RPE) cells attached to gelatin microcarriers (GM) produces long-term motor improvement in humans with Parkinson’s disease (PD). Sustained activity has also been demonstrated in both rodent and monkey models of PD with post-mortem histological evidence of microcarrier-attached RPE cells. Recently, cell labeling methods have been described that allow the in vivo follow-up of implanted cells through non-invasive imaging methods such as magnetic resonance imaging (MRI). We describe here the safety and efficacy of labeling RPE cells with dextran-coated superparamagnetic iron oxide (SPIO) nanoparticles for future in vivo MR imaging. Feridex I.V. (a ferumoxide solution composed of a dextran coated SPIO nanoparticle suspension), in combination with the transfection agent protamine sulfate (Pro), is used as a pre-labeling agent that is taken up by RPE cells in vitro. Using an immortalized, commercial RPE cell-line (RPE-19), SPIO-labeled RPE-19 cells were assessed for short-term viability and proliferative activity using the trypan blue exclusion method, and compared to standard unlabeled RPE-19 cells in normal culture conditions. In vitro samples of SPIO-labeled RPE-19 cells attached to GM were then imaged on a Phillips 3T MR scanner to assess SPIO-labeling efficacy. Results showed no differences in proliferative activity or cell viability up to 10 days post-seeding between SPIO-labeled and non-labeled RPE-19 cells. Differences in MR signal intensity was demonstrated using different Pro concentrations, different RPE-19 cell concentrations, and was confirmed by Prussian Blue histochemistry. These results demonstrate the potential use of SPIO-labeling for the in vivo follow-up of cell implants.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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